Anti-MCM3 Rabbit Polyclonal Antibody for WB, IP, ELISA - P25205

Core Product Specifications and Parameters

Biological Background: MCM3 Function and Localization

• MCM3 (DNA replication licensing factor MCM3), also designated HCC5, P1.h, RLFB, or P1-MCM3, is a core component of the hexameric MCM2-7 complex.
• As part of the MCM2-7 helicase and the CDC45-MCM-GINS (CMG) complex, MCM3 is essential for DNA replication initiation and elongation, enforcing once-per-cell-cycle genome duplication (PubMed:32453425, PubMed:34694004, PubMed:34700328). Related references: PMID:32453425 PMID:34694004 PMID:34700328
• The active ATPase sites within the MCM2-7 ring are formed at subunit interfaces, where MCM3 provides a conserved arginine finger motif critical for ATP hydrolysis and helicase processivity (PubMed:32453425). Related references: PMID:32453425
• MCM3 is required for entry into S phase and for cell division, playing a central role in cell cycle progression.
• The protein localizes predominantly in the nucleus and associates with chromosomes.
• Post-translational modifications include acetylation and phosphorylation; it contains functional nucleotide-binding (ATP), DNA-binding, and helicase domains.
• The molecular mass of MCM3 is approximately 91 kDa (UniProt: 90981 Da).

Experimental Guidance and Technical Tips

• The immunogen encompasses the N-terminal region (amino acids 1–295) of human MCM3; consider this when designing epitope-based assays.
• For western blot (WB) and immunoprecipitation (IP), perform a dilution series to optimize the polyclonal antibody concentration in your experimental system.
• Verify cross-reactivity with mouse and rat samples using appropriate tissue or cell lysates.
• Ensure the antibody is validated in your specific ELISA format; preliminary checkerboard titrations are recommended.
• As with all polyclonal antibodies, lot-to-lot consistency should be monitored when transitioning to production-scale workflows.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research teams with high-quality IVD raw materials, including validated polyclonal antibodies like this MCM3 rabbit pAb. Our supply network covers optimized antibody pairs, bulk ancillary reagents, and rare-target sourcing, supporting your DNA replication and cell cycle–focused assay development. From concept to clinic, CamelBio streamlines procurement so you can accelerate project timelines with confidence.