Anti-EZH2/KMT6 Rabbit Polyclonal Antibody for WB, IP, ELISA - Q15910

Core Product Specifications and Parameters

Biological Background: EZH2/KMT6 Function and Localization

• EZH2 is the catalytic subunit of the PRC2/EED-EZH2 Polycomb group complex, methylating histone H3 at Lys-9 and Lys-27 to mediate transcriptional repression.
  Related references: PMID:14532106, PMID:15225548, PMID:15385962
• The enzyme can mono-, di-, and tri-methylate H3K27, contributing to dynamic chromatin regulation.
  Related references: PMID:15231737, PMID:17210787, PMID:18285464
• Displays a preference for unmethylated H3K27 substrates, with decreasing activity as methylation increases (H3K27me0 > H3K27me1 > H3K27me2).
  Related references: PMID:22323599, PMID:30923826
• Plays a critical role in embryonic stem cells by establishing H3K27me3 marks necessary for stem cell identity and proper differentiation.
  Related references: PMID:19026781
• Recruits DNA methyltransferases, linking histone methylation to DNA methylation and reinforcing gene silencing.
  Related references: PMID:16357870, PMID:17200670
• Acts as a transcriptional repressor of key developmental and tumor suppressor genes, including HOXC8, HOXA9, MYT1, and CDKN2A.
  Related references: PMID:16179254, PMID:18086877, PMID:20935635
• Extends its regulatory function by methylating non-histone proteins such as GATA4 and RORA.
  Related references: PMID:23063525
• Expressed in many tissues, with overexpression observed in various cancers; detected in ovary primordial follicles and oocytes.
  Related references: PMID:14532106, PMID:31451685

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fusion protein corresponding to amino acids 158-261 of human EZH2 (NP_001190176.1). This N‑terminal region may detect full‑length and certain truncated isoforms; epitope mapping can further define binding specificity.
• This rabbit polyclonal antibody is validated for Western blotting (WB), immunoprecipitation (IP), and ELISA. In WB, a specific band at ~85 kDa is expected; optimize antibody dilution and blocking conditions for the target sample type.
• Cross‑reactivity with human and mouse suggests a conserved epitope. If working with other species, confirm reactivity experimentally.
• EZH2 is a nuclear protein; use appropriate nuclear extraction or fixation/permeabilization protocols for immunodetection applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio acts as a comprehensive IVD raw-material partner, providing validated antibodies, proteins, and ancillary reagents to accelerate diagnostic and research workflows. For epigenetic and oncology programs targeting EZH2, we offer this high-quality rabbit polyclonal antibody alongside optimized antibody pairs and bulk recombinant proteins. Our sourcing capabilities ensure consistent access to both common and rare targets, supporting your projects from early development through clinical translation.