Anti-N-Myc/MYCN Rabbit Polyclonal Antibody for WB, IF-P, IHC-P, ELISA - P04198

Core Product Specifications and Parameters

Biological Background: N-Myc/MYCN Function and Localization

• The N-Myc/MYCN protein, encoded by the MYCN gene (also known as BHLHE37 or NMYC), belongs to the class E basic helix-loop-helix (bHLH) family of transcription factors.
• It acts as a transcriptional activator, positively regulating the transcription of MYCNOS in neuroblastoma cells.
• MYCN localizes predominantly to the nucleus, consistent with its role in DNA binding and transcription regulation.
• Tissue expression is prominent in neuronal cells of the cerebrum, as well as in neuroblastomas and thyroid tumors at the protein level.
• As a proto-oncogene, MYCN is implicated in oncogenic transformation; its activity is regulated by phosphorylation.
• The protein has a calculated molecular weight of approximately 50 kDa, corroborating the observed band size in Western blot applications.

Experimental Guidance and Technical Tips

• The antibody was raised against a recombinant fusion protein corresponding to amino acids 168-267 of human N-Myc/MYCN (NP_005369.2), a region potentially conserved across species, supporting its cross-reactivity with mouse and rat.
• Recommended for WB, IF-P, IHC-P, and ELISA; consider optimizing antibody dilution and detection protocols for each assay system.
• Validated in human, mouse, and rat; for other species, consult sequence homology of the immunogen region.
• Note that MYCN is a low-abundance transcription factor; enrichment strategies or sensitive detection methods may enhance signal in certain sample types.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic developers and research laboratories streamlined access to high-quality IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies like this anti-N-Myc/MYCN polyclonal antibody, and bulk ancillary reagents. With expertise across the entire development pipeline—from concept to clinic—CamelBio also supports rare target raw-material sourcing and provides technical consulting to accelerate your assay development. For oncology and neurobiology research applications, rely on CamelBio as your procurement bridge to reliable, scalable reagent supply.