Anti-MCM6 Rabbit Monoclonal Antibody (KD Validated) for WB, ELISA - Q14566

Core Product Specifications and Parameters

Biological Background: DNA replication licensing factor MCM6 Function and Localization

• DNA replication licensing factor MCM6 (p105MCM) is a core component of the MCM2-7 complex, which acts as the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells.
• MCM6, together with other MCM proteins, forms the CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication and serves as the foundation for replisome assembly (PubMed:16899510, PubMed:32453425, PubMed:34694004). Related references: PMID:16899510, PMID:32453425, PMID:34694004
• The active ATPase sites in the MCM2-7 ring are formed at subunit interfaces; a conserved arginine finger motif from one subunit is provided in trans to the ATP-binding site of the adjacent subunit, and these six ATPase sites contribute differentially to helicase activity (PubMed:32453425). Related references: PMID:32453425
• MCM6 is predominantly localized in the nucleus during G1 phase and associates with chromatin, consistent with its role in DNA replication licensing.
• The protein undergoes various post-translational modifications including acetylation and phosphorylation, which may regulate its activity during the cell cycle.
• According to UniProt, MCM6 is classified under keywords such as ATP-binding, Cell cycle, DNA replication, DNA-binding, Glycoprotein, Helicase, Hydrolase, Nucleotide-binding, Phosphoprotein, and Reference proteome.

Experimental Guidance and Technical Tips

• Since the immunogen corresponds to amino acids 601-821, the antibody recognizes a C-terminal region of human MCM6; consider validating detection of any relevant cleavage products or isoforms in your experimental system.
• For WB, a positive control such as HeLa or MCF-7 cell lysate (human) or NIH/3T3 lysate (mouse) is recommended; optimize antibody dilution and blocking conditions for your specific sample.
• The antibody has been used for knockout/knockdown validation (KD Validated), indicating reliable detection of endogenous MCM6; treat this as supporting specificity data.
• For ELISA, assess the antibody’s performance as a capture or detection reagent; pair with a suitable anti-MCM6 antibody or use recombinant MCM6 protein as standard.
• The expected band size is ~93 kDa; use appropriate gel percentage (e.g., 8% SDS-PAGE) and molecular weight markers for clear separation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio delivers one-stop access to high-quality IVD raw materials, including validated antibody pairs and optimized monoclonal antibodies like this anti-MCM6 clone, essential for DNA replication and cell cycle research. We support diagnostic manufacturers and labs with bulk ancillary reagents and custom sourcing for rare targets, ensuring seamless procurement from concept to clinic. Explore our portfolio to find complementary products such as other MCM complex antibodies and nuclei acid binding protein reagents that integrate smoothly into your assay development.