Anti-SIX3 Rabbit Polyclonal Antibody for WB and ELISA - O95343

Core Product Specifications and Parameters

Biological Background: Homeobox protein SIX3 Function and Localization

• SIX3 is a homeobox transcriptional regulator that binds to the ATTA core sequence, acting as both a repressor and activator of target gene expression.
• In forebrain development, SIX3 represses WNT1 to allow zona limitans intrathalamica formation, ensuring proper anterior-posterior diencephalon patterning.
• SIX3 directly activates SHH expression in the ventral midline of the rostral diencephalon, and SHH signalling reciprocally maintains SIX3 expression.
• The protein is required for telencephalon formation and postnatal maturation of radial glia into ependymal cells.
• During eye development, SIX3 activates PAX6 in the lens ectoderm and suppresses WNT8B in the anterior neural plate, essential for lens induction and neuroretina formation.
• Mutations in SIX3 are associated with holoprosencephaly, a severe brain malformation (keyword: Disease variant).
• Localized to the nucleus, SIX3 participates in Wnt/β-catenin and BMP signaling pathways during development (keywords: Nucleus, Repressor, Developmental protein, Homeobox).

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the C-terminal region (aa 250-336) of human SIX3; consider sequence alignment when using cross-reactive species to confirm epitope conservation.
• For Western blot, a band at approximately 35 kDa is expected in human, mouse, and rat samples; validate loading controls and use appropriate tissue lysates (e.g., embryonic brain or eye tissues).
• In ELISA, the immunogen peptide may serve as a positive control; optimize coating conditions and use blocking buffers suitable for polyclonal antibodies.
• Verify specificity by including a negative control (e.g., SIX3 knockout cell lysate or peptide blocking) and testing multiple antibody concentrations.
• For immunofluorescence or other applications not listed, note that this antibody is only validated for WB and ELISA; additional optimization may be required.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement for diagnostic manufacturers and labs by offering a comprehensive portfolio of validated antibodies, antigens, and ancillary reagents. This anti-SIX3 polyclonal antibody supports research on forebrain development, eye formation, and neural differentiation—areas where reliable immunodetection tools are critical. Beyond this product, CamelBio can source rare-target antibodies, bulk reagents, and customized assay components to accelerate your project from concept to clinical application.