MCM5 Rabbit Monoclonal Antibody for WB, IHC-P, IP, ELISA - P33992

Core Product Specifications and Parameters

Biological Background: MCM5 Function and Localization

• DNA replication licensing factor MCM5 is a core component of the MCM2-7 complex, which functions as the replicative helicase essential for the initiation and elongation of DNA replication in eukaryotic cells.
  Related references:
  PMID:40940420

• MCM5 is an integral subunit of the CDC45-MCM-GINS (CMG) helicase complex, the molecular machine responsible for unwinding template DNA at the replication fork during S phase.
  Related references:
  PMID:16899510
  PMID:32453425
  PMID:34694004

• The ATPase activity of the MCM2-7 ring is contributed by six active sites formed at subunit interfaces, with a conserved arginine finger motif provided in trans, although these sites contribute differentially to helicase function.
  Related references:
  PMID:32453425

• Located in the nucleus and associated with chromosomes, MCM5 plays a critical role in ensuring once-per-cell-cycle DNA replication by participating in the pre-replication complex assembly.

• Post-translational modifications of MCM5 include phosphorylation, acetylation, and ubiquitin conjugation, which regulate its function and protein interactions within the replication licensing system.

• MCM5 is also known by aliases CDC46 and P1-CDC46, reflecting its homology to yeast cell division cycle proteins, and belongs to the MCM family of ATP-dependent helicases.

• Mutations in MCM5 have been associated with Meier-Gorlin syndrome 8 (MGORS8), a disorder characterized by primordial dwarfism and microcephaly, underscoring its essential role in cell proliferation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 635-734 within the C-terminal region of human MCM5 (P33992); this epitope may be accessible in both denaturing and native conditions.
• For western blotting, an expected band around 82 kDa should be observed in whole-cell lysates from human, mouse, or rat cell lines; include appropriate molecular weight markers and loading controls.
• In immunohistochemistry, optimization of antigen retrieval methods and primary antibody dilution is recommended for formalin-fixed, paraffin-embedded tissues to achieve specific nuclear staining.
• The antibody may be effective for immunoprecipitation of MCM5 and co-immunoprecipitation of interacting MCM complex members; validate with known interaction partners.
• For ELISA, consider using the antibody as a capture or detection reagent against recombinant MCM5 or cell lysates; pairing with a non-overlapping clone may improve sandwich assay performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for diagnostic manufacturers and research institutions, offering one-stop access to IVD raw materials, technical support, and consultation from concept to clinic. For DNA replication and cell cycle research targets like MCM5, we supply validated monoclonal antibodies, optimized antibody pairs, bulk ancillary reagents, and specialized sourcing for rare targets. Partner with CamelBio to streamline your procurement and accelerate your assay development.