Anti-MCM7 Rabbit Monoclonal Antibody for WB, IF/ICC, IP, ELISA - P33993

Core Product Specifications and Parameters

Biological Background: MCM7 Function and Localization

• MCM7 is a core subunit of the MCM2-7 replicative helicase complex, which is essential for the 'once per cell cycle' initiation and elongation of DNA replication in eukaryotic cells. Related references: PMID:25661590, PMID:32453425, PMID:34694004
• As part of the CDC45-MCM-GINS (CMG) helicase, MCM7 contributes to the unwinding of template DNA at replication forks, forming the core of the replisome. Related references: PMID:25661590, PMID:32453425, PMID:34694004
• The ATPase active sites within the MCM2-7 ring are formed by neighboring subunits, with a critical arginine finger motif provided in trans; individual active sites likely contribute differentially to helicase activity. Related references: PMID:32453425
• MCM7 is required for S-phase checkpoint activation in response to UV-induced DNA damage, highlighting its role in genome integrity maintenance.
• The protein localizes to the nucleus and associates with chromatin, consistent with its function in DNA replication.
• MCM7 undergoes multiple post-translational modifications, including phosphorylation, acetylation, and ubiquitination, which regulate its activity and cell cycle progression.
• The human MCM7 protein has a predicted molecular weight of approximately 81 kDa and is also known by alternative names such as CDC47 homolog and P1.1-MCM3.

Experimental Guidance and Technical Tips

• The immunogen for this antibody is a synthetic peptide from amino acids 600-700 of human MCM7, a region predicted to be accessible and suitable for diverse immunoassays.
• For Western blotting, expect a band at approximately 81 kDa in human cell lysates; nuclear extract enrichment may improve sensitivity.
• In immunofluorescence/ICC, anticipate nuclear localization; co-staining with replication foci markers (e.g., PCNA) can confirm S-phase specific patterns.
• For immunoprecipitation, this rabbit monoclonal antibody can capture the MCM complex; note that MCM7 forms stable interactions with other MCM2-7 subunits.
• Validated for ELISA, the antibody may be adapted for quantitative detection; consider appropriate coating buffers and blocking agents.
• As reactivity is confirmed for human, cross-reactivity with other species should be verified experimentally if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated partner for IVD raw material sourcing, serving diagnostic manufacturers, research labs, and clinical institutions from concept to commercialization. For DNA replication and cell cycle research, we provide validated monoclonal antibodies such as this anti-MCM7 clone, along with optimized antibody pairs, bulk ancillary reagents, and expert sourcing for rare targets. Rely on CamelBio's comprehensive supply chain to accelerate your assay development and ensure consistent, high-quality materials.