Anti-TRAF6 Rabbit Polyclonal Antibody for IF/ICC and ELISA - Q9Y4K3

Core Product Specifications and Parameters

Biological Background: TRAF6 Function and Localization

• TRAF6 is an E3 ubiquitin ligase that, together with UBE2N and UBE2V1, catalyzes the synthesis of Lys-63-linked polyubiquitin chains on target proteins such as IRAK1, AKT1, and IKBKG, and also ubiquitinates free polyubiquitin to trigger MAP3K7 activation. Related references: PMID:11057907, PMID:18347055, PMID:19465916, PMID:19675569
• Through its ubiquitin ligase activity, TRAF6 activates the NF-kappa-B and JUN signaling pathways, playing a critical role in inflammatory and immune responses. Related references: PMID:16378096, PMID:17135271, PMID:17703191
• TRAF6 serves as an adapter protein in signal transduction cascades initiated by TNF receptor, IL-1 receptor, and IL-17 receptor, linking receptor engagement to downstream kinase activation. Related references: PMID:12140561, PMID:19825828, PMID:8837778
• The protein is localized in the cytoplasm, cell cortex, nucleus, and lipid droplets, reflecting its diverse functions in spatial signaling.
• TRAF6 is widely expressed in tissues including heart, brain, placenta, lung, liver, skeletal muscle, kidney, and pancreas, consistent with its broad role in cellular regulation.
• Structurally, TRAF6 contains a RING finger domain, zinc-finger motifs, and coiled-coil regions; it is classified as a transferase involved in ubiquitin conjugation pathways and processes such as osteogenesis, immunity, and DNA damage response.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 50–150 of human TRAF6, a region that may contain conserved motifs; cross-reactivity with mouse and rat TRAF6 is reported, making this antibody a candidate for rodent studies.
• For immunofluorescence/immunocytochemistry, optimal fixation and permeabilization conditions should be determined, as TRAF6 localizes to multiple subcellular compartments (cytoplasm, nucleus, lipid droplets). A nuclear counterstain may help confirm localization patterns.
• In ELISA applications, the polyclonal nature of the antibody may provide robust signal detection; titrations and standard curves are recommended for quantitative assays.
• Given TRAF6’s role in immune signaling, testing the antibody in stimulated versus unstimulated cells (e.g., with IL-1β, TNFα, or RANKL) may reveal activation-dependent changes in expression or localization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop sourcing partner for IVD raw materials, offering a broad range of high-quality antibodies, recombinant proteins, and ancillary reagents to support every stage of diagnostic development. This anti-TRAF6 polyclonal antibody, validated for applications such as IF/ICC and ELISA, is just one example of our commitment to providing reliable tools for immune signaling research and assay development. Whether you need validated antibody pairs, custom antibody generation, or bulk raw materials, CamelBio is here to streamline your supply chain and accelerate your projects from concept to clinic.