Anti-MCM3 Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - P25205

Core Product Specifications and Parameters

Biological Background: MCM3 Function and Localization

• MCM3 is a DNA replication licensing factor, also known as DNA polymerase alpha holoenzyme-associated protein P1, P1-MCM3, RLF subunit beta, or p102.
• It is a core component of the MCM2-7 complex, which functions as the replicative helicase essential for once-per-cell-cycle DNA replication initiation and elongation.
• MCM3 is a subunit of the CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication and around which the replisome is built. Related references: PMID:32453425 PMID:34694004 PMID:34700328
• The active ATPase sites in the MCM2-7 ring are formed by interaction surfaces of neighboring subunits, with a conserved arginine finger motif provided in trans to the ATP-binding site of the adjacent subunit. The six ATPase active sites contribute differentially to helicase activity. Related references: PMID:32453425
• MCM3 is required for entry into S phase and for cell division.
• Subcellular localization is primarily nuclear, associated with chromosomes.
• The protein is subject to acetylation, alternative splicing, phosphorylation, and glycosylation, as indicated by UniProt keywords.
• It has a molecular weight of approximately 91 kDa (calculated 90,981 Da from UniProt).

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1-100 of human MCM3. For western blot, expect a band around 91 kDa. Validate in the relevant sample system.
• For IHC-P and IF/ICC, consider antigen retrieval and validation in appropriate tissue samples.
• For IP and ELISA, use standard protocols and confirm specificity in human, mouse, or rat samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive access to IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. This anti-MCM3 antibody supports research in DNA replication and cell cycle regulation. Our sourcing network ensures timely procurement for diagnostic manufacturers and research laboratories.