Anti-RPL6 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q02878

Core Product Specifications and Parameters

Biological Background: RPL6 Function and Localization

• RPL6, also known as 60S ribosomal protein L6 or TaxREB107, is a core component of the large 60S ribosomal subunit, a ribonucleoprotein complex responsible for cellular protein synthesis.
  Related references: PMID:12962325 PMID:23636399 PMID:25901680
• (Microbial infection) RPL6 specifically binds to domain C of the Tax-responsive enhancer element in the HTLV-I long terminal repeat, indicating a role in regulating viral gene expression.
  Related references: PMID:8457378
• The protein localizes to the cytoplasm, including the cytosol and rough endoplasmic reticulum, consistent with its involvement in ribosomal assembly and function.
• RPL6 undergoes post-translational modifications such as acetylation, phosphorylation, and ubiquitination (Ubl conjugation), as identified by proteomic analyses.
• It has been designated as neoplasm-related protein C140, suggesting potential relevance to cancer biology.
• The gene encoding RPL6, also termed TXREB1, highlights its dual role in translation and transcriptional regulation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a region spanning amino acids 188-288 of human RPL6, which may provide target specificity; consider this when designing complementary detection tools.
• For Western blot (WB), the predicted molecular weight is approximately 33 kDa; validate band specificity using appropriate positive controls such as HeLa or HEK293 cell lysates.
• In immunofluorescence (IF/ICC), confirm the expected cytoplasmic and endoplasmic reticulum localization; include appropriate controls to assess specificity.
• For ELISA, optimize coating antigen concentrations and antibody dilutions based on the assay format; cross-reactivity data (human, mouse, rat) can guide species selection for sample testing.
• Although reactivity with human, mouse, and rat is reported, always verify performance in your specific experimental system and sample type.

CamelBio: Your One-Stop Sourcing Bridge

At CamelBio, we provide diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies like this anti-RPL6 antibody, as well as bulk ancillary reagents and rare target raw-material sourcing for protein synthesis and ribosomal research. Contact us to explore how we can support your next project.