Anti-MCM5 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - P33992

Core Product Specifications and Parameters

Biological Background: MCM5 Function and Localization

• MCM5 is a DNA replication licensing factor, essential for 'once per cell cycle' DNA replication initiation and elongation as a component of the MCM2-7 complex. Related references: PMID:40940420
• Acts as a core component of the CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication. Related references: PMID:16899510 PMID:32453425 PMID:34694004
• The active ATPase sites in the MCM2-7 ring involve interaction surfaces between neighboring subunits, with a critical arginine finger motif provided in trans. Related references: PMID:32453425
• Subcellular localization is primarily nuclear, associated with chromatin.
• Alternative names include CDC46 homolog, P1-CDC46, and MGORS8. Mutations are linked to Meier-Gorlin syndrome (MGORS8) and dwarfism.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitination (reflected by keywords such as Acetylation, Phosphoprotein, Ubl conjugation).
• Keywords categorize MCM5 as an ATP-binding, DNA-binding helicase involved in cell cycle and DNA replication, with proteomics identification and disease variant annotations.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal region (aa 1-230) of human MCM5, potentially detecting full-length and truncated isoforms; validate specificity with proper controls.
• For Western blot (WB), the predicted molecular weight is ~82 kDa; confirm band size using positive control lysates from human, mouse, or rat cells.
• Species cross-reactivity is stated for human, mouse, and rat; verify performance in your sample system if using other species.
• For immunohistochemistry (IHC-P), consider antigen retrieval protocols; for immunofluorescence (IF/ICC), permeabilization and blocking steps should be optimized.
• For immunoprecipitation (IP), antibody titration and pre-clearing steps may be necessary to reduce background.
• ELISA can be used for detection; include appropriate positive and negative controls to ensure assay sensitivity and specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with comprehensive access to IVD raw materials, including validated antibody pairs and optimized polyclonal and monoclonal antibodies. Our MCM5 antibody offerings serve DNA replication and cell cycle research, with bulk and ancillary reagent supply capabilities. For rare targets or custom sourcing needs, CamelBio bridges the gap from concept to clinic.