Anti-MCM2 Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P49736

Core Product Specifications and Parameters

Biological Background: MCM2 Function and Localization

• DNA replication licensing factor MCM2 (minichromosome maintenance protein 2 homolog; also known as BM28, CCNL1, CDCL1) is a core component of the MCM2-7 complex, which is the putative replicative helicase.
• The MCM2-7 complex forms the core of the CDC45-MCM-GINS (CMG) helicase, the molecular machine responsible for unwinding template DNA during replication, and functions in both initiation and elongation phases (PubMed:32453425, PubMed:34694004, PubMed:34700328). Related references: PMID:32453425 PMID:34694004 PMID:34700328
• Active ATPase sites in the MCM2-7 ring are formed at the interface of neighboring subunits, with a conserved arginine finger motif provided in trans relative to the ATP-binding site of the adjacent subunit (PubMed:32453425). Related references: PMID:32453425
• MCM2 is required for entry into S phase and for cell division (PubMed:8175912). Related references: PMID:8175912
• Plays a role in the development of terminally differentiated hair cells in the cochlea and induces cell apoptosis (PubMed:26196677). Related references: PMID:26196677
• Localizes to the nucleus and associates with chromosomes during DNA replication.
• Post-translational modifications include phosphorylation, acetylation, and ubiquitination; the protein contains zinc-finger motifs and exhibits ATP-binding and DNA-binding activities.
• Mutations in MCM2 are associated with autosomal dominant non-syndromic hearing loss (DFNA70).

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–100) of human MCM2. Consider using the recommended peptide blocking strategy for specificity control in relevant assays.
• For Western blot (WB), expect a band at approximately 102 kDa. Optimize reducing/denaturing conditions and use appropriate loading controls.
• For immunohistochemistry (IHC-P), antigen retrieval methods (e.g., heat-induced epitope retrieval) may be required to expose the epitope; validate with known positive and negative tissue controls.
• For immunofluorescence/immunocytochemistry (IF/ICC), fixation and permeabilization conditions should be empirically determined, as the nuclear localization of MCM2 may require optimized access.
• For ELISA, coating with the immunogenic peptide may serve as a positive control; confirm binding under your specific assay conditions.
• Although cross-reactivity with mouse and rat is reported, we recommend verifying specificity in your experimental system, as epitope conservation may vary.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your comprehensive one-stop platform for IVD raw materials, supporting diagnostic manufacturers, laboratories, and research institutes with antibodies, antigens, and ancillary reagents tailored to gene regulation and cell proliferation targets like MCM2. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk reagents, streamlining your development from concept to clinic. Whether you need rare target sourcing or large-scale production, CamelBio is committed to bridging your supply needs for DNA replication and cell cycle research.