Anti-MCU Polyclonal Antibody for WB, IF/ICC, ELISA - Q8NE86

Core Product Specifications and Parameters

Biological Background: MCU Function and Localization

• MCU (Calcium uniporter protein, mitochondrial) is the channel-forming subunit of the mitochondrial inner membrane calcium uniporter complex (uniplex), mediating calcium uptake into the mitochondrial matrix. This process is critical for ATP production, cytoplasmic calcium signaling, and activation of cell death pathways. Related references: PMID:21685886, PMID:21685888, PMID:22822213
• MCU channel activity is tightly regulated by the calcium-sensor subunits MICU1 and MICU2 (or MICU3 in certain tissues), which set the threshold for calcium entry and prevent calcium overload at low cytosolic concentrations. Related references: PMID:24560927, PMID:26903221, PMID:30454562
• Located in the mitochondrial inner membrane, MCU is a multi-pass membrane protein with a coiled-coil domain and several transmembrane helices.
• In cardiomyocytes, MCU buffers the amplitude of systolic calcium rises and acts as a key regulator of short-term mitochondrial calcium loading during acute stress, mediating the ‘fight-or-flight’ response. Related references: PMID:22822213, PMID:25603276
• In pancreatic beta-cells, MCU regulates glucose-dependent insulin secretion by controlling mitochondrial calcium uptake. Related references: PMID:22829870, PMID:22904319
• MCU is subject to various post-translational modifications, including acetylation, phosphorylation, and glutathionylation, and contains a predicted coiled-coil domain essential for its function.

Experimental Guidance and Technical Tips

• For Western blotting, a band around 40 kDa may be observed in whole-cell lysates from human, mouse, or rat cells. Use reducing conditions and validate with appropriate controls.
• For immunofluorescence/immunocytochemistry, optimize fixation and permeabilization protocols to preserve mitochondrial morphology while allowing antibody access to the inner membrane.
• For ELISA applications, consider direct coating with the recombinant immunogen or capture of native MCU from tissue extracts; validate the assay with relevant species samples.
• As a rabbit polyclonal antibody, it may provide multiple epitope recognition, enhancing signal in detection applications. Lot-to-lot consistency should be verified for critical experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research laboratories to provide one-stop access to high-quality IVD raw materials, including validated antibodies, recombinant antigens, and ancillary reagents. This anti-MCU polyclonal antibody supports mitochondrial calcium signaling research and can be integrated into immunoassay development. Our extensive supply network covers bulk and rare target sourcing, helping you accelerate from concept to clinic.