Anti-MAP3K11 Rabbit Polyclonal Antibody for WB, ELISA - Q16584

Core Product Specifications and Parameters

Biological Background: MAP3K11 Function and Localization

• MAP3K11, also known as Mixed lineage kinase 3 (MLK3), is a serine/threonine-protein kinase that functions as a mitogen-activated protein kinase kinase kinase (MAP3K) upstream of the JNK and p38 signaling pathways.
• It activates MAP2K4/MKK4 and MAP2K7/MKK7, which in turn phosphorylate MAPK8/JNK1, MAPK14/p38, and MAPK3/ERK, thereby regulating cell proliferation, differentiation, and stress responses.
• Required for serum-stimulated cell proliferation and plays a role in mitogen-stimulated phosphorylation and activation of BRAF, although it does not phosphorylate BRAF directly.
• Influences microtubule organization during the cell cycle and localizes to the centrosome and microtubule organizing center in the cytoplasm.
• Widely expressed across fetal and adult tissues, including lung, liver, heart, kidney, placenta, skeletal muscle, pancreas, and brain.
• Contains an SH3 domain and undergoes alternative splicing, yielding multiple isoforms with potentially distinct functions (Keywords: Alternative splicing, SH3 domain, ATP-binding, Phosphoprotein).
• Subject to phosphorylation, which modulates its kinase activity and downstream signaling, contributing to its role as a key node in mitogen and cytokine signaling.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a region within the N-terminal domain (aa 140-260) of human MAP3K11; this sequence is distinct from the kinase domain and may be conserved across isoforms. Cross-reactivity with mouse MAP3K11 suggests sequence conservation; validation in mouse cell lines or tissues is recommended.
• For Western blotting, the predicted molecular weight is ~93 kDa, but alternative splicing or post-translational modifications could result in additional bands; include appropriate controls and verify with siRNA or knockout lysates where possible.
• In ELISA, the polyclonal nature may provide robust signal, but optimize coating antigen and antibody dilution to minimize non-specific binding.
• Given MAP3K11's role in signaling pathways, stimulation of cells with serum or cytokines before lysis may enhance detection, especially when assessing activation states.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio bridges the gap between discovery and clinical translation by offering a comprehensive range of IVD raw materials, including validated antibodies, protein reagents, and customized sourcing services. The MAP3K11 rabbit polyclonal antibody underscores our capability in the kinase signaling and cell proliferation research arena. Whether you need bulk antibodies, matched detection pairs, or rare target reagents, our team can facilitate efficient procurement and technical support.