Anti-MAP3K1 Rabbit Polyclonal Antibody for WB, IF, IHC, ELISA - Q13233

Core Product Specifications and Parameters

Biological Background: MAP3K1 Function and Localization

• Mitogen-activated protein kinase kinase kinase 1 (MAP3K1) is also known as MEKK1 or MEKK, and functions as a key component of intracellular signaling cascades.
• It activates the ERK and JNK kinase pathways by phosphorylating MAP2K1 and MAP2K4, respectively (PubMed:9808624). Related references: PMID:9808624
• MAP3K1 may directly phosphorylate MAPK8/JNK1 kinase, linking it to diverse cellular responses (PubMed:17761173). Related references: PMID:17761173
• The protein also activates the NF-kappa-B pathway by phosphorylating CHUK and IKBKB, central regulators of immune and inflammatory responses (PubMed:9808624). Related references: PMID:9808624
• MAP3K1 features an ATP-binding domain, zinc-finger motifs, and is subject to regulatory post-translational modifications including acetylation and phosphorylation.
• With a molecular weight of approximately 164 kDa, MAP3K1 is a large multidomain kinase that integrates signals from various stimuli.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 1400-1512 within the C-terminal kinase domain of human MAP3K1; antibody binding may be influenced by protein conformation or post-translational modifications in this region.
• For Western blot detection, note the large predicted molecular weight (~164 kDa); use appropriate electrophoresis conditions (e.g., low-percentage resolving gels) and efficient transfer protocols.
• In immunofluorescence and immunohistochemistry applications, fixation and permeabilization steps should be optimized for your specific sample type to ensure proper epitope exposure.
• Reported cross-reactivity with mouse and rat MAP3K1 makes this antibody suitable for studies in common model organisms; however, we recommend confirming specificity with appropriate controls in each experimental system.
• For ELISA-based assays, the antibody can be employed as either a capture or detection reagent; optimal concentrations and buffer conditions should be determined empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material supplier, connecting diagnostic manufacturers and research laboratories with validated antibodies, recombinant proteins, and ancillary reagents. Our MAP3K1 antibody supports investigations into kinase signaling pathways, cancer biology, and immunological research. We offer one-stop access to rare target raw materials, bulk production options, and technical consultation to accelerate your assay development from initial concept to clinical application.