Anti-MEKK3 (MAP3K3) Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q99759

Core Product Specifications and Parameters

Biological Background: MEKK3 Function and Localization

• MEKK3, encoded by the MAP3K3 gene, is a serine/threonine-protein kinase that functions as a mitogen-activated protein kinase kinase kinase in intracellular signaling cascades.
• It plays a critical role in the activation of transcription factors NF-kappa-B, AP-1, and DDIT3, linking extracellular stimuli to transcriptional responses.
• MEKK3 belongs to the MAP kinase kinase kinase family and participates in the MAPK signaling pathway, regulating processes such as inflammation, stress responses, and cell differentiation.
• The protein is subject to alternative splicing, generating multiple isoforms that may have distinct functional properties.
• MEKK3 contains an ATP-binding domain and requires magnesium for its catalytic activity, characteristic of protein kinases.
• Phosphorylation regulates its activity, and it has been identified through proteomics as a phosphoprotein.
• Mutations in MAP3K3 are associated with disease variants, highlighting its importance in human pathology.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-220), which may detect full-length MEKK3 and some isoforms in Western blotting; expect a band near 71 kDa, with possible additional bands due to modifications or alternative splicing.
• For Western blotting, it is advisable to validate the antibody in target-relevant cell lines (e.g., HEK293T, HeLa) and use appropriate loading controls.
• In immunohistochemistry (paraffin), consider antigen retrieval methods (e.g., citrate buffer, pH 6.0) and optimize primary antibody dilution for your specific tissue type.
• For ELISA, the antibody can be tested as a capture or detection reagent; pairing with a monoclonal anti-MEKK3 antibody may enhance sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive one-stop sourcing for IVD raw materials, including validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and rare target procurement. This anti-MEKK3 antibody supports research in MAP kinase signaling, transcription regulation, and disease pathology; CamelBio can also assist in sourcing complementary reagents and assemblies for assay development and diagnostics manufacturing.