Anti-MEK5 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q13163

Core Product Specifications and Parameters

Biological Background: MEK5 (MAP2K5) Function and Localization

• MEK5, also known as MAP2K5, is a dual specificity mitogen-activated protein kinase kinase that can phosphorylate both serine/threonine and tyrosine residues.
• It acts as a scaffold for the formation of a ternary signaling complex involving MAP3K2/MAP3K3, MAP3K5, and MAPK7 (ERK5), playing a key role in the MAPK/ERK signaling cascade.
• Activation of this pathway is critical for protecting cells from stress-induced apoptosis, promoting neuronal survival, and supporting cardiac development and angiogenesis.
• In cardiomyocytes, MEK5 functions as a negative regulator of apoptosis by facilitating STUB1/CHIP-mediated ubiquitination and degradation of ICER-type isoforms of CREM (By similarity).
• The protein is broadly expressed in adult tissues, with particularly high abundance in heart and skeletal muscle.
• MEK5 undergoes post-translational modifications including acetylation and phosphorylation, which may regulate its activity and interactions.
• The gene undergoes alternative splicing, potentially generating multiple isoforms with distinct functional properties.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-190) of human MEK5; thus, the antibody may recognize isoforms sharing this sequence.
• For Western blotting, the expected band size is approximately 50 kDa; validate with appropriate positive and negative controls.
• In immunohistochemistry and immunofluorescence, heart and skeletal muscle tissues are suitable positive controls given the high expression of MEK5.
• Cross-reactivity with mouse and rat allows use in rodent experimental models; however, perform species-specific validation.
• For ELISA, consider using recombinant MEK5 protein as a standard to optimize assay conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with validated antibody pairs, monoclonal/polyclonal antibodies, and bulk ancillary reagents for MAPK signaling targets. For MEK5-related projects, we offer one-stop access to IVD raw materials from concept to clinic, including rare target sourcing and technical consulting. Streamline your cardiac development or apoptosis research with our reliable supply chain and expert support.