Anti-MEK3 Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - P46734

Core Product Specifications and Parameters

Biological Background: MEK3 Function and Localization

• Dual specificity protein kinase that phosphorylates threonine and tyrosine residues on p38 MAP kinases (MAPK11, MAPK12, MAPK13, MAPK14), thereby activating downstream stress response pathways.
• Activated by pro-inflammatory cytokines and environmental stressors such as UV irradiation, osmotic shock, and heat shock.
• Functions as part of a signaling cascade initiated by adrenergic receptor ADRA1B, leading to activation of MAPK14.
• Belongs to the MAP kinase kinase family and is also known as MAPKK3, MKK3, PRKMK3, or SAPKK2.
• Abundantly expressed in skeletal muscle but widely distributed across many tissues, reflecting its central role in stress signaling.
• Subject to post-translational modifications including acetylation and phosphorylation, with multiple phosphotyrosine and phosphoserine sites documented.
• Alternative splicing generates multiple isoforms; the canonical isoform has a molecular weight of approximately 39 kDa.

Experimental Guidance and Technical Tips

• The immunogen spans the catalytic kinase domain (residues 150-347), making this antibody likely to recognize both phosphorylated and unphosphorylated forms; verify specificity using phosphatase-treated lysates in WB.
• For WB, a band at ~39 kDa is expected; consider using a molecular weight marker for accurate sizing.
• In IF/ICC, permeabilize cells adequately and include a no-primary control to assess background staining.
• For ELISA, the antibody can be used for capture or detection; validate with recombinant MEK3 protein or cell lysates from stimulated cells.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a comprehensive one-stop platform for IVD raw materials, technical services, and consulting. This anti-MEK3 monoclonal antibody is part of our extensive portfolio of validated reagents for cellular signaling and kinase pathway research. Beyond single antibodies, we offer matched antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets, enabling seamless assay development from concept to clinic.