Anti-MEKK3 Monoclonal Antibody for WB - Q99759

Core Product Specifications and Parameters

Biological Background: MEKK3 Function and Localization

• MEKK3, also known as MAP3K3 or MAPKKK3, is a 626-amino acid serine/threonine kinase with a predicted molecular weight of approximately 71 kDa.
• It is a member of the MAP kinase kinase kinase (MAP3K) family, which activates downstream MAP2K and MAPK in signaling cascades.
• MEKK3 mediates the activation of transcription factors NF-kappa-B, AP1, and DDIT3, linking extracellular signals to gene expression changes involved in immunity, stress responses, and apoptosis.
• The kinase domain binds ATP and requires divalent metal ions such as magnesium for catalytic activity.
• MEKK3 is subject to regulatory phosphorylation, and its dysregulation has been associated with disease variants (as indicated by keywords).
• Multiple isoforms are generated by alternative splicing, potentially contributing to functional diversity.

Experimental Guidance and Technical Tips

• The antibody is validated for western blot (WB) and ELISA. For WB, consider using whole cell lysates from human or mouse cell lines known to express MEKK3, and optimize primary antibody concentration empirically.
• For ELISA, the antibody can be used as either capture or detection antibody after appropriate titration; compatibility with specific assay formats should be tested.
• The immunogen corresponds to the N-terminal region (amino acids 1-100), suggesting potential utility in detecting total MEKK3 or specific isoforms; however, validation in the relevant sample system is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive portfolio of IVD raw materials, including validated monoclonal antibodies like anti-MEKK3, for manufacturers and researchers working on cell signaling and kinase-related diseases. We support your development pipeline with optimized antibody pairs, bulk ancillary reagents, and expert sourcing of rare-target materials—all backed by our one-stop technical and consulting services.