Anti-MAP4K3 Rabbit Polyclonal Antibody for WB and ELISA - Q8IVH8

Core Product Specifications and Parameters

Biological Background: MAP4K3 Function and Localization

• MAP4K3 (Mitogen-activated protein kinase kinase kinase kinase 3) is a serine/threonine kinase, also known as germinal center kinase-related protein kinase (GLK), MEK kinase kinase 3 (MEKKK 3), MAPKKKK3, and RAB8IPL1.
• It responds to environmental stress and acts upstream of the JUN N-terminal (JNK) signaling pathway. Related references: PMID:9275185
• Functions as an activator of the Hippo signaling pathway; it is partially redundant with STK3/MST2 and STK4/MST1 in phosphorylating LATS1/2, contributing to organ size control and tumor suppression. Related references: PMID:26437443
• Ubiquitously expressed, with highest levels in heart, brain, placenta, skeletal muscle, kidney, and pancreas; lower in lung and liver.
• Contains conserved kinase domains and is regulated by acetylation and phosphorylation events, as indicated by keywords ATP-binding, phosphoprotein, and acetylation.
• Alternative splicing generates multiple isoforms, potentially diversifying its functional roles.
• The immunogen maps to amino acids 755–894, lying within the C-terminal region of the protein, which may contribute to isoform specificity.

Experimental Guidance and Technical Tips

• The antibody is a polyclonal reagent raised against a recombinant C-terminal fragment (aa 755–894); consider verifying specificity via knockdown or blocking peptide experiments in your sample system.
• For Western blotting, the predicted molecular weight is approximately 101 kDa; use a gel percentage and transfer conditions suitable for high-molecular-weight proteins and include a pre-stained marker for reference.
• ELISA protocols may require optimization of antibody dilution and blocking; titrate the antibody using a known positive control.
• Cross-reactivity is demonstrated for human, mouse, and rat; validate in the specific tissue or cell type of interest to confirm species-specific performance.
• Due to the polyclonal nature, lot-to-lot variation can occur; it is advisable to reserve a reference lot for long-term studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive, one-stop access to high-quality IVD raw materials, supporting diagnostic manufacturers and researchers from early development to clinical application. This MAP4K3 polyclonal antibody is part of our extensive kinase research portfolio, and we can assist with sourcing validated antibody pairs, optimized monoclonal antibodies, bulk ancillary reagents, and even rare target raw-material procurement. Whether you need a single reagent or a tailored supply solution, our expertise bridges the gap between your ideas and the clinic.