Anti-MEP1A Polyclonal Antibody for WB and ELISA - Q16819

Core Product Specifications and Parameters

Biological Background: MEP1A (Meprin A Subunit Alpha) Function and Localization

• MEP1A encodes the alpha subunit of meprin A, a zinc-dependent metalloprotease belonging to the astacin family of proteases.
• The protein is also known as endopeptidase-2, PABA peptide hydrolase (PPH alpha), and N-benzoyl-L-tyrosyl-P-amino-benzoic acid hydrolase subunit alpha.
• Meprin A is a membrane-bound enzyme, anchored to the cell surface via a single-pass transmembrane domain.
• The precursor form is a zymogen that requires proteolytic removal of the pro-peptide for catalytic activation.
• Structurally, MEP1A contains an EGF-like domain, multiple disulfide bonds, and is heavily glycosylated, existing as a proteoglycan.
• Based on UniProt annotation, key features include hydrolase activity, metal-binding, and zinc coordination, with a molecular weight of approximately 84.4 kDa.

Experimental Guidance and Technical Tips

• For Western blotting, consider using 8–10% SDS-PAGE to optimize separation of the ~84 kDa MEP1A protein.
• As a membrane protein, efficient extraction may require detergents such as Triton X-100 or NP-40 in the lysis buffer; validate conditions in your sample system.
• When performing ELISA, titrate both coating antigen and primary antibody to establish optimal working concentrations; antigen may be recombinant MEP1A or cell/tissue lysates.
• The antibody is reported to cross-react with mouse and rat MEP1A; however, always confirm cross-reactivity in your specific tissue or cell types before routine use.
• Because this is a polyclonal antibody, lot-to-lot variability should be assessed and controlled in critical quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with streamlined access to IVD raw materials, technical services, and consulting—from concept to clinic. This anti-MEP1A rabbit polyclonal antibody exemplifies our commitment to supplying validated antibodies for metalloprotease research and assay development. Beyond this product, we offer ready-to-use matched antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets to accelerate your IVD pipeline.