Anti-MAP3K4 Polyclonal Antibody for WB, IHC-P, ELISA - Q9Y6R4

Core Product Specifications and Parameters

Biological Background: MAP3K4 Function and Localization

• Encoded by the MAP3K4 gene (also known as MTK1, MEKK4, MAPKKK4), this protein is a mitogen-activated protein kinase kinase kinase that functions upstream of stress-activated pathways.
• Its primary role is to act as a component of protein kinase signal transduction cascades, specifically activating the p38 and JNK MAP kinase pathways, but not the ERK pathway.
• Directly phosphorylates and activates the dual-specificity kinases MAP2K4 (MKK4) and MAP2K6 (MKK6), leading to subsequent phosphorylation of p38 and JNK.
• Localizes predominantly to the cytoplasm, with enrichment in the perinuclear region, consistent with its role in signaling hub formation.
• Displays tissue-specific expression, with high levels in heart, placenta, skeletal muscle, and pancreas, and lower expression in other tissues.
• Belongs to the serine/threonine-protein kinase family, containing an ATP-binding pocket and requiring magnesium as a cofactor for catalytic activity.
• Subject to alternative splicing and phosphorylation, indicating potential regulatory modifications that influence its function and interaction networks.

Experimental Guidance and Technical Tips

• The immunogen was raised against a synthetic peptide from the N-terminal region (amino acids 1–100). This may influence the detection of isoforms or cleavage products; verify specificity in relevant biological samples.
• For Western blot (WB), the predicted molecular weight is ~182 kDa; adequate separation and transfer conditions for high-molecular-weight proteins should be optimized.
• In IHC-P, antigen retrieval conditions and tissue fixation protocols may require optimization to achieve optimal signal-to-noise ratios.
• Cross-reactivity with rat has been observed, which can be useful for cross-species studies; however, validate the antibody’s performance in your specific model system.
• For ELISA, the antibody may be used as a capture or detection reagent; consider titration and pairing with appropriate secondary antibodies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers, labs, and research institutes with comprehensive access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This MAP3K4 antibody exemplifies our growing portfolio of kinase-related reagents, ideal for researchers investigating MAP kinase signaling. Whether you need validated antibody pairs, bulk ancillary reagents, or rare-target sourcing, CamelBio streamlines your procurement for accelerated assay development.