Anti-MMP3 Polyclonal Antibody for WB, ELISA - P28862

Core Product Specifications and Parameters

Biological Background: MMP3 (Stromelysin-1) Function and Localization

• MMP3 (Stromelysin-1) is a secreted zinc-dependent metalloproteinase of the matrix metalloproteinase family, synthesized as an inactive zymogen and activated extracellularly by proteolytic cleavage.
• It exhibits broad substrate specificity, degrading fibronectin, laminin, gelatins, collagens (types III, IV, X, IX), and cartilage proteoglycans, playing a crucial role in extracellular matrix turnover.
• MMP3 activates other pro-MMPs like MMP9 and processes growth factors and plasminogen, thereby regulating ECM remodeling and signaling pathways.
• Primarily localized to the extracellular space and matrix, MMP3 can also function intracellularly in specialized contexts, such as promoting dopaminergic neuron degeneration via microglial activation and alpha-synuclein cleavage (PubMed:17116747).
  Related references: PMID:17116747
• It contributes to innate immunity with antiviral activity; upon viral challenge, MMP3 translocates to the nucleus and modulates NF-kappa-B to inhibit viral replication (PubMed:35940311).
  Related references: PMID:35940311
• Key structural features include zinc- and calcium-binding sites, disulfide bonds, and hemopexin-like repeats essential for substrate recognition and inhibition.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (385-477) of human MMP3, which shares high homology with mouse MMP3. For Western blot, consider using reducing and non-reducing conditions to detect both pro- and active forms, as MMP3 can form disulfide-linked multimers.
• For ELISA applications, validate the antibody's reactivity against recombinant or purified mouse MMP3 protein, as cross-reactivity is reported.
• Consider using appropriate controls (e.g., untreated vs. PMA-stimulated fibroblast lysates) to verify MMP3 induction and specificity in mouse models.
• The predicted molecular weight of mouse MMP3 is ~54 kDa (pro-form); processing may generate lower molecular weight active forms, so ensure adequate membrane transfer and blocking for detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and researchers one-stop access to IVD raw materials, technical services, and consulting. This MMP3 polyclonal antibody supports studies in extracellular matrix biology, inflammation, and neurology. CamelBio provides validated antibody pairs, optimized polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to bridge your workflow from concept to clinic.