Anti-MAPK1/MAPK3 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P28482 / P27361

Core Product Specifications and Parameters

Biological Background: MAPK1/MAPK3 Function and Localization

• MAPK1 (ERK2, p42-MAPK) and MAPK3 (ERK1, p44-MAPK) are the two prototypic mitogen-activated protein kinases (MAPKs) that constitute the core of the conserved MAPK/ERK signaling cascade.
• Upon activation, MAPK1/3 phosphorylate a broad range of nuclear and cytoplasmic substrates—including transcription factors (e.g., ELK1, FOS), cytoskeletal proteins, and other kinases—to regulate cell growth, proliferation, differentiation, adhesion, and survival.
• MAPK1 specifically phosphorylates GLI1 in response to smoothened signaling, promoting GLI1 dissociation from SUFU and its nuclear translocation, thereby positively regulating the Hedgehog pathway. Related references: PMID:35831023
• Under hypoxic conditions, MAPK1 phosphorylates phosphoglycerate kinase PGK1, directing it to mitochondria and suppressing acetyl-coenzyme A production from pyruvate. Related references: PMID:26942675
• The kinase shuttles between the cytoplasm and nucleus, localizing to focal adhesions, caveolae, the cytoskeleton (including centrosomes and spindles), and membrane-associated structures depending on the cellular context.
• MAPK1 kinase activity is tightly regulated by phosphorylation and dephosphorylation events; it serves as a hub for signal propagation, itself phosphorylating downstream kinases such as RSK, MSK, MNK, and MAPKAP kinases.
• Post-translational modifications include activating phosphorylation at Thr and Tyr residues in the activation loop, acetylation, and ubiquitination, which modulate its stability, localization, and interaction partners.
• Knockout or aberrant MAPK1/3 expression and signaling are implicated in cancer, neurological disorders, and other diseases; the kinase is targeted by host-virus interactions.

Experimental Guidance and Technical Tips

• This rabbit polyclonal antibody was raised against a synthetic peptide corresponding to amino acids 200-300 of human MAPK1 and MAPK3 (ERK2 and ERK1); it is predicted to recognize both kinases and may detect multiple bands in western blot due to isoforms and phospho-forms.
• For western blotting, load 20-30 µg of total protein lysate and use appropriate controls (e.g., serum-starved vs. growth factor-stimulated cells) to observe ERK activation. Expected molecular weights range from 36 to 43 kDa.
• In immunofluorescence/ICC, fix cells with 4% paraformaldehyde and permeabilize with 0.1% Triton X-100; primary antibody incubation overnight at 4°C is recommended. Validate staining specificity with peptide blocking or siRNA knockdown.
• For ELISA, this polyclonal antibody can be used as a capture or detection reagent; optimal coating and detection conditions should be determined empirically. A matched monoclonal antibody pair may improve sensitivity and specificity.
• The antibody cross-reacts with mouse and rat MAPK1/MAPK3; however, perform a species-specific validation in your experimental system to confirm reactivity and signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated one-stop sourcing partner for IVD raw materials, from concept to clinic. This MAPK1/MAPK3 polyclonal antibody exemplifies our commitment to providing high-quality immunological reagents for diagnostic development. Beyond antibodies, we supply validated antibody pairs, recombinant antigens, bulk ancillary reagents, and custom sourcing solutions to accelerate your assay development in signal transduction and oncology research.