Anti-MAPK11 (p38β/SAPK2b) Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q15759

Core Product Specifications and Parameters

Biological Background: MAPK11 Function and Localization

• MAPK11, also known as p38β or stress-activated protein kinase 2b (SAPK2b), is a member of the p38 mitogen-activated protein kinase (MAPK) family. It is a serine/threonine kinase that serves as a critical component of the MAP kinase signal transduction pathway, responding to extracellular stimuli like pro-inflammatory cytokines and physical stress. Related references: PMID:12452429, PMID:20626350, PMID:35857590

• MAPK11 functions are largely redundant with MAPK14 (p38α), and it is estimated to phosphorylate 200–300 substrates. Downstream targets include kinases such as MAPKAPK2 (MK2) and MAPKAPK3 (MK3), which regulate post-transcriptional gene expression, and RPS6KA5 (MSK1) and RPS6KA4 (MSK2), which directly phosphorylate transcription factors (e.g., CREB1, ATF1, NF-κB) and histone H3, contributing to chromatin remodeling and immediate-early gene induction. Related references: PMID:9687510, PMID:11154262, PMID:10330143, PMID:15356147, PMID:9430721

• In the nucleus, p38 MAPKs phosphorylate numerous transcription factors including ATF2, ATF6, ELK1, TP53, MEF2C, and MEF2A, thereby modulating gene expression. They also regulate chromatin modifiers and remodelers, as evidenced by p38-dependent enrichment of histone H3 phosphorylation at Ser10 (H3S10ph) at promoters of inflammatory genes like IL6, IL8, and IL12B, facilitating NF-κB recruitment. Related references: PMID:10330143, PMID:15356147, PMID:9430721

• MAPK11 is involved in the regulation of protein turnover and ectodomain shedding. It phosphorylates CFLAR to regulate proteasome-mediated degradation, and phosphorylates ADAM17 to promote shedding of TGF-α family ligands, thus activating EGFR signaling.

• MAPK11 phosphorylates NLRP1 downstream of MAP3K20/ZAK in response to UV-B irradiation and ribosome collisions, promoting NLRP1 inflammasome activation and pyroptosis. Related references: PMID:35857590

• MAPK11 phosphorylates the methyltransferase DOT1L at multiple serine and threonine residues (Ser-834, Thr-900, Ser-902, Thr-984, Ser-1001, Ser-1009, Ser-1104), potentially impacting chromatin modifications. Related references: PMID:38270553

• MAPK11 is localized in both the cytoplasm and nucleus and exhibits highest expression in brain and heart, with additional expression in placenta, lung, liver, skeletal muscle, kidney, and pancreas.

• The protein undergoes alternative splicing and is regulated by phosphorylation. It belongs to the protein kinase superfamily and is involved in stress responses, transcription regulation, and signal transduction.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 264-364 of human MAPK11. This region is likely conserved across human, mouse, and rat, supporting the reported cross-reactivity. Consider using this antibody to detect total MAPK11 regardless of phosphorylation state.
• For Western blot, a signal at approximately 41 kDa is expected. Appropriate positive controls include lysates from cells stimulated with UV-B, cytokines, or osmotic stress to confirm MAPK11 expression and activation.
• In immunofluorescence/ICC, the antibody may detect MAPK11 in both cytoplasm and nucleus. Optimize permeabilization conditions and validate specificity using appropriate negative controls.
• For ELISA, the antibody can be used for detection of MAPK11 in cell extracts or purified samples. Pairing with a suitable capture antibody may enable sandwich ELISA development; perform titration and cross-reactivity assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with streamlined access to high-quality IVD raw materials like this Anti-MAPK11 polyclonal antibody, supporting MAP kinase signaling research and assay development. Our portfolio spans validated antibody pairs, monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. From concept to clinic, CamelBio is your trusted partner in IVD raw material procurement.