Anti-MAP3K8 Rabbit Polyclonal Antibody for WB, ELISA - P41279

Core Product Specifications and Parameters

Biological Background: MAP3K8 Function and Localization

• MAP3K8 is a serine/threonine-protein kinase, also known as COT (Cancer Osaka thyroid oncogene), TPL-2 (Tumor progression locus 2), and proto-oncogene c-Cot. Isoform 1 shows some transforming activity, though weaker than the oncogenic variant.
• Required for lipopolysaccharide (LPS)-induced, TLR4-mediated activation of the MAPK/ERK pathway in macrophages, thus critical for production of the pro-inflammatory cytokine TNF during immune responses.
• Involved in regulation of T-helper cell differentiation and IFNG expression in T-cells; mediates host resistance to bacterial infection through negative regulation of type I interferon (IFN) production.
• Transduces CD40 and TNFRSF1A signals that activate ERK in B-cells and macrophages, and thus may play a role in the regulation of immunoglobulin production. May also transduce TNF signals that activate JNK and NF-kappa-B in some cell types.
• Subcellular localization is primarily cytoplasmic.
• According to UniProt keywords, the protein is an ATP-binding magnesium-dependent phosphoprotein, highlighting its regulation by phosphorylation and its role as a proto-oncogene kinase.
• Expressed in several normal tissues and human tumor-derived cell lines.
• In adipocytes, activates MAPK/ERK pathway in an IKBKB-dependent manner in response to IL1B and TNF, leading to induction of lipolysis. Plays a role in the cell cycle.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1-100 of human MAP3K8 (NP_005195.2). Consider verifying the antibody's reactivity with reported isoforms or splice variants.
• For Western blot (WB), the predicted protein weight is ~53kDa. Validate endogenous expression levels in the relevant sample system, such as LPS-stimulated macrophages or T-cells.
• Cross-reactivity is reported for human, mouse, and rat. Confirm species-specific binding under your experimental conditions.
• ELISA application can be explored with appropriate optimization of coating antigen and detection conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting. This anti-MAP3K8 rabbit polyclonal antibody is offered as part of a comprehensive portfolio covering immune signaling and cancer research targets. In addition to validated antibody pairs and optimized polyclonal antibodies, we support bulk procurement of ancillary reagents and can assist in sourcing rare-target raw materials to accelerate your diagnostic development from concept to clinic.