Anti-MAP3K4 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - Q9Y6R4

Core Product Specifications and Parameters

Biological Background: MAP3K4 Function and Localization

• MAP3K4, also known as MEKK4 or MTK1, is a serine/threonine-protein kinase that functions as a key mediator of stress-activated mitogen-activated protein kinase (MAPK) signaling cascades.
• It specifically activates the JNK and p38 MAP kinase pathways by phosphorylating and activating MAP2K4 (MKK4) and MAP2K6 (MKK6), but does not activate the ERK pathway.
• The protein localizes predominantly to the cytoplasm, with enrichment in the perinuclear region.
• MAP3K4 is expressed at high levels in heart, placenta, skeletal muscle, and pancreas, with lower expression in other tissues, suggesting tissue-specific regulatory roles.
• Its kinase activity requires ATP and magnesium ions, consistent with its classification as a serine/threonine kinase.
• Alternative splicing has been reported, potentially giving rise to multiple isoforms with distinct regulatory properties.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a region within amino acids 100–200 of human MAP3K4; thus, the antibody may recognize both full-length and truncated forms if the epitope is conserved. Validate in the relevant sample system.
• For Western blotting (WB), a band around 182 kDa is expected; consider using an appropriate protein ladder and optimizing transfer conditions for high molecular weight proteins.
• For immunohistochemistry (IHC-P), antigen retrieval and antibody dilution should be optimized per tissue type; expression levels may vary depending on tissue and experimental conditions.
• For ELISA, the antibody may be used as a capture or detection reagent; cross-reactivity with mouse and rat orthologs is reported, so validate in those species if applied.
• As MAP3K4 is involved in stress signaling, consider treating cells with appropriate stimuli (e.g., anisomycin, UV) to enhance expression or phosphorylation before analysis.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents for targets in the MAPK signaling pathway. We also assist in sourcing rare-target raw materials, ensuring your project advances efficiently from research to regulatory approval.