Anti-MEKK2 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - Q9Y2U5

Core Product Specifications and Parameters

Biological Background: MEKK2 Function and Localization

• MEKK2 (also known as MAP3K2 or MAPKKK2) is a serine/threonine-protein kinase that functions as a mitogen-activated protein kinase kinase kinase (MAP3K).
• It is a key component of protein kinase signal transduction cascades, regulating the JNK and ERK5 pathways by phosphorylating and activating downstream MAP2K5 (MEK5) and MAP2K7 (MKK7) (By similarity).
• MEKK2 plays a role in caveolae kiss-and-run dynamics, suggesting involvement in membrane trafficking and signal compartmentalization.
• Localizes to both the cytoplasm and nucleus, enabling it to transmit signals from membrane receptors to nuclear targets.
• Contains ATP-binding and kinase domains, requiring magnesium for catalytic activity.
• Identified as a phosphoprotein, indicating regulation by phosphorylation events.
• Ubiquitination (Ubl conjugation) may regulate its stability or activity.
• Belongs to the serine/threonine protein kinase family and is part of the reference proteome.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the N-terminal region (aa 1-100) of human MEKK2; this region may be less conserved, potentially contributing to specificity—validate in your experimental system.
• For Western blot, the predicted molecular weight is ~70 kDa (Q9Y2U5); ensure proper loading controls and consider phosphatase inhibitors if studying phosphorylation events.
• IHC-P applications should include appropriate antigen retrieval and positive/negative tissue controls; optimize detection conditions for your sample type.
• ELISA performance depends on proper coating and detection conditions; consider direct or indirect formats and appropriate blocking steps.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with this high-quality anti-MEKK2 rabbit monoclonal antibody, suitable for multiple immunoassays. As your one-stop sourcing partner, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents to support your MAP kinase pathway research and IVD development. Contact us for rare target raw-material sourcing and technical consulting.