Anti-MAPK1/MAPK3 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P28482 / P27361

Core Product Specifications and Parameters

Biological Background: MAPK1/MAPK3 (ERK1/2) Function and Localization

• MAPK1, also known as ERK2 or p42-MAPK, is a serine/threonine kinase that, together with MAPK3 (ERK1), constitutes the core of the MAPK/ERK signaling cascade, governing critical cellular processes such as proliferation, differentiation, and apoptosis.
• The MAPK/ERK pathway phosphorylates over 160 substrates, including transcription factors (e.g., ELK1, FOS), cytoskeletal regulators, and other kinases, thereby relaying extracellular signals to the nucleus and various organelles. Related references: PMID:35831023
• MAPK1 localizes to multiple subcellular compartments—cytoplasm, nucleus, cytoskeleton, focal adhesions, and mitotic spindle— underscoring its dynamic roles in processes such as mitosis and cell adhesion.
• Activation of MAPK1 requires dual phosphorylation of threonine and tyrosine residues in its activation loop; it is also subject to acetylation, ubiquitination, and other post-translational modifications that fine-tune its activity.
• Under hypoxic conditions, MAPK1 phosphorylates phosphoglycerate kinase PGK1, promoting its mitochondrial translocation to suppress acetyl-coenzyme A formation from pyruvate. Related references: PMID:26942675
• Beyond its kinase-dependent signaling, MAPK1 can function as a transcriptional repressor, binding specific DNA motifs to inhibit interferon-gamma-induced gene expression.
• Given its central role in signaling, MAPK1/ERK2 is implicated in cancer, developmental disorders, and host-virus interactions, making it a valuable biomarker and research target.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide derived from amino acids 200–300 of human MAPK1/MAPK3, a region highly conserved among ERK1/ERK2 orthologs in human, mouse, and rat, which likely underpins the observed cross-reactivity. Check sequence alignment if applying to other species.
• In Western blotting, multiple bands may be observed due to phosphorylation-induced shifts and the presence of splice variants; the predicted molecular weight range is approximately 36–43 kDa. Verify with positive controls and, if needed, phosphatase treatment.
• For immunohistochemistry (IHC-P), antigen retrieval conditions (e.g., citrate buffer, pH 6.0) are often required; include relevant positive and negative tissue controls to confirm specificity.
• When used in ELISA, titrate the antibody to determine optimal coating and detection concentrations. Consider sandwich ELISA formats using matched antibody pairs for higher sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers and research labs from concept through clinical application. This anti-MAPK1/MAPK3 antibody is part of our extensive portfolio of validated antibodies for cell signaling research. We also provide complementary services such as custom monoclonal/polyclonal antibody development, bulk ancillary reagents, and rare target sourcing to accelerate your assay development and commercialization.