Anti-HLTF Rabbit Monoclonal Antibody for WB, ELISA - Q14527

Core Product Specifications and Parameters

Biological Background: HLTF Function and Localization

• HLTF (Helicase-like transcription factor) is a multifunctional protein combining DNA-dependent ATPase, E3 ubiquitin-protein ligase, and chromatin remodeling activities, encoded by the HLTF gene (also known as HIP116A, RNF80, SMARCA3, SNF2L3, ZBU1). It plays critical roles in DNA damage tolerance, epigenetic regulation, and transcription. Related references: PMID:18316726 PMID:18719106 PMID:26051180
• As a key effector in post-replication repair, HLTF catalyzes 'Lys-63'-linked polyubiquitination of monoubiquitinated PCNA at 'Lys-164' upon genotoxic stress, promoting error-free template switching to bypass DNA lesions. Related references: PMID:18316726 PMID:18719106
• HLTF acts as an epigenetic regulator by binding histone H3 trimethylated at 'Lys-9' (H3K9me3) and mediating histone H3 'Lys-23' polyubiquitination (H3K23ub), which serves as a docking site for DNMT1, thereby ensuring replication-coupled DNA methylation maintenance. Related references: PMID:40680746
• It exhibits ATP-dependent dsDNA translocase activity without classical helicase activity, mediating replication fork reversal by unwinding and annealing nascent and parental strands to suppress DNA synthesis and maintain genomic stability. Related references: PMID:1994885
• HLTF cooperates with MSH2 to resolve G-quadruplex (G4) DNA structures, limiting replication stress and G4 accumulation throughout the cell cycle. Related references: PMID:39142279
• It contributes to nucleotide excision repair by evicting lesion-containing oligonucleotides using its HIRAN domain, and can also displace single-stranded DNA from triplex structures through ATP-dependent translocation. Related references: PMID:26051180 PMID:31960921
• Subcellular localization is predominantly nuclear and chromosome-associated, consistent with its roles in DNA repair and chromatin dynamics.
• Expressed in multiple tissues including brain, heart, kidney, liver, lung, pancreas, placenta, and skeletal muscle. The protein is subject to alternative initiation, ATP-binding, metal-binding (zinc-finger), methylation, and phosphorylation, reflecting its regulation by multiple post-translational modifications.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 900-1009 of human HLTF, a region selected for specificity and likely accessible in denaturing conditions. For WB, consider using whole-cell lysates or nuclear extracts from human, mouse, or rat cell lines, as cross-reactivity has been reported in these species.
• Due to the large predicted molecular weight (~114 kDa), ensure adequate protein separation by using appropriate gel percentages and extended transfer times during western blotting.
• For ELISA, the antibody may be used as a capture or detection reagent; consider pairing with a validated matched antibody if developing a sandwich assay, and optimize coating conditions for the synthetic peptide or recombinant target.
• Since HLTF undergoes multiple post-translational modifications (ubiquitination, phosphorylation, methylation), band shifts may be observed; validate the antibody's ability to detect modified forms in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted procurement hub for IVD raw materials, offering diagnostic manufacturers and researchers streamlined access to validated antibodies, bulk ancillary reagents, and rare-target raw materials. This Anti-HLTF Rabbit Monoclonal Antibody supports investigation into DNA damage repair, chromatin remodeling, and epigenetic regulation—areas of active diagnostic biomarker research. For scientists developing assays in these fields, CamelBio can source complementary reagents such as matched antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary supplies to accelerate your project from concept to clinic.