Anti-von Willebrand Factor (VWF) Rabbit Monoclonal Antibody for WB - P04275

Core Product Specifications and Parameters

Biological Background: von Willebrand Factor Function and Localization

• von Willebrand factor (VWF) is a large multimeric glycoprotein encoded by the VWF gene (gene ID 7450); it circulates in plasma and is essential for hemostasis.
• It mediates platelet adhesion to subendothelial collagen at sites of vascular injury by bridging the extracellular matrix and the platelet GPIb-IX-V receptor complex.
• VWF also acts as a carrier and stabilizer for coagulation factor VIII, protecting it from premature proteolytic degradation and clearance.
• The protein is secreted and localizes to the extracellular space and extracellular matrix; plasma is the primary site of its presence.
• Key structural features include multiple disulfide bonds, repeat domains, and extensive glycosylation, contributing to its high molecular weight (~309 kDa).
• Alternative splicing produces various isoforms, and mutations in VWF are associated with von Willebrand disease, a bleeding disorder.
• Post-translational modifications include cleavage on basic residues and secretion via a signal peptide.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1150–1300 of human VWF, a conserved region that may explain the observed cross-reactivity with mouse.
• For Western blot, the predicted molecular weight is 309 kDa; consider using a low-percentage gel and optimized transfer conditions for high-molecular-weight proteins.
• In ELISA experiments, plasma or recombinant VWF may serve as suitable positive controls; validate performance in your specific sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research labs with comprehensive IVD raw material solutions, including validated antibodies like this anti-VWF monoclonal. Our portfolio covers hemostasis and coagulation targets, offering bulk supply and custom sourcing for rare reagents. Partner with us to streamline your assay development from concept to clinic.