Anti-Transferrin Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P02787

Core Product Specifications and Parameters

Biological Background: Transferrin Function and Localization

• Serotransferrin (also known as Beta-1 metal-binding globulin, Siderophilin, or simply Transferrin) is an iron-binding transport protein encoded by the TF gene, capable of binding two Fe³⁺ ions in association with an anion, typically bicarbonate.
• It is responsible for the transport of iron from sites of absorption and heme degradation to sites of storage and utilization, and may also stimulate cell proliferation.
• The protein is secreted and expressed by the liver, then released into the plasma.
• Transferrin is a glycoprotein containing disulfide bonds and can be modified by phosphorylation and methylation.
• During microbial infection, pathogens such as Neisseria species and the parasite Trypanosoma brucei can capture transferrin to extract iron for their own use.
• It possesses a characteristic bilobal structure with repeated domains, as indicated by its 3D structure and the "Repeat" keyword.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning residues 50–150 of human Transferrin. Ensure the target epitope is accessible in your specific sample preparation and assay conditions.
• For Western blot, the observed molecular weight should be approximately 77 kDa under reducing conditions.
• Consider using appropriate positive and negative controls for IHC-P, such as liver tissue sections where Transferrin is highly expressed.
• Verify cross-reactivity with other species if required, as the antibody is validated for human reactivity.
• For ELISA, optimize coating buffers and detection conditions to maintain the conformational integrity of the antigen.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a complete sourcing solution for IVD raw materials, specializing in iron metabolism and infection research tools like this anti-Transferrin monoclonal antibody. Our one-stop platform supplies validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents, supporting diagnostic manufacturers and labs from concept to clinical application.