Anti-Mouse IgM Rabbit Monoclonal Antibody for WB, IF, IHC, ELISA - P01872

Core Product Specifications and Parameters

Biological Background: Mouse IgM Function and Localization

• Immunoglobulin heavy constant mu (Ighm) encodes the constant region of mouse IgM heavy chains, existing as secreted (sIgM) and membrane-bound (mIgM) isoforms via alternative splicing.
• Secreted IgM multimerizes into pentamers and hexamers, providing high-avidity antigen binding. Related references: PMID:10899913, PMID:28135254, PMID:34788614
• sIgM mediates effector functions through Fc receptor FCMR binding and complement activation via C1q, promoting phagocytosis of pathogens and apoptotic cells. Related references: PMID:10899913, PMID:11062505, PMID:28135254
• Membrane-bound IgM is a component of the B cell receptor (BCR) complex, providing tonic signals for B cell survival and regulating allelic exclusion during development.
• Expressed in bone marrow and at the protein level in B-1 and B-2 cells.
• Contains immunoglobulin domains, disulfide bonds, and N-glycosylation sites typical of antibody constant regions.
• Natural IgM is polyreactive and recognizes conserved structures, while immune IgM is produced upon pathogen exposure and is antigen-specific. Related references: PMID:10899913, PMID:28135254, PMID:34788614

Experimental Guidance and Technical Tips

• The immunogen (aa 105-454 of mouse Ighm) targets the IgM constant region; the antibody may detect both secreted and membrane-bound forms.
• For Western blot, consider reducing SDS-PAGE to observe the ~50 kDa heavy chain; under non-reducing conditions, high-molecular-weight multimers may be visible.
• Validate specificity in relevant sample systems, such as mouse B-cell lysates or lymphoid tissues, and confirm cross-reactivity with rat samples where applicable.
• For IF and IHC, optimize fixation/permeabilization protocols; use IgM-positive controls like spleen or lymph node sections.
• For ELISA, the antibody may serve as a capture or detection reagent for mouse IgM; titrate to determine optimal working concentration.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. This anti-mouse IgM rabbit monoclonal antibody supports B cell research and humoral immunity studies, complementing our comprehensive portfolio of immunoglobulin detection reagents. We assist diagnostic manufacturers in accelerating assay development from concept to clinic, including rare target sourcing and technical consultation.