Anti-TLR2 Rabbit Monoclonal Antibody for WB, IF/ICC, IF-P, ELISA - O60603

Core Product Specifications and Parameters

Biological Background: TLR2 Function and Localization

• TLR2 (Toll-like receptor 2), also known as CD282 and TIL4, is a leucine-rich repeat-containing receptor with a TIR domain that forms disulfide bonds and undergoes glycosylation and ubiquitin-like conjugation.
• TLR2 cooperates with LY96 and TLR1 or TLR6 to recognize bacterial lipoproteins and lipopeptides, initiating signaling via MYD88 and TRAF6 that activates NF-kB, promoting inflammatory cytokine secretion and possibly apoptosis. Related references: PMID:17889651, PMID:21078852, PMID:10426995, PMID:10426996
• TLR2 forms ligand-dependent activation clusters with CD14 and CD36, such as TLR2:TLR6:CD14:CD36 for diacylated lipopeptides and TLR2:TLR1:CD14 for triacylated lipopeptides, which signal from the cell surface and traffic to the Golgi. Related references: PMID:16880211
• TLR2 cooperatively recognizes mycoplasmal MALP-2, tuberculosis factor STF, phenol-soluble modulin, and Borrelia OspA lipoprotein with TLR6. Related references: PMID:11441107
• TLR2 serves as a receptor for M.tuberculosis lipoproteins LprA, LprG, LpqH and PstS1, often requiring coreceptors TLR1, CD14 or CD36, to modulate antigen-presenting cell functions. Related references: PMID:19362712
• TLR2 mediates M.tuberculosis HSP70 (dnaK)-induced NF-kB activation, and M.tuberculosis PstS1 and bacterial peptidoglycan trigger p38 MAPK and ERK1/2 signaling through this receptor. Related references: PMID:15809303, PMID:16622205
• Subcellularly, TLR2 is found on the cell membrane, in cytoplasmic vesicles, on phagosome membranes, and within membrane rafts.
• TLR2 is highly expressed in peripheral blood leukocytes, especially monocytes, and also in bone marrow, lymph node, spleen, lung, and fetal liver.

Experimental Guidance and Technical Tips

• The immunogen comprises the full-length extracellular domain (aa 1–784), which may elicit antibodies recognizing native TLR2 in diverse assay conditions.
• For Western blot, a band around 90 kDa is expected; validate expression in TLR2-positive cell lines or activated monocytes.
• In immunofluorescence (IF/ICC, IF-P), consider using permeabilization protocols suitable for membrane and phagosome-localized proteins.
• For ELISA, the antibody may be used for direct detection or as a capture/detection pair; validate with recombinant TLR2 protein or cell lysates.
• Cross-reactivity with human and mouse TLR2 suggests suitability for translational studies; consider validation in the relevant model system.

CamelBio: Your One-Stop Sourcing Bridge

As a one-stop sourcing partner for IVD raw materials, CamelBio offers validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents for toll-like receptor and innate immunity research. This TLR2 Rabbit mAb complements our portfolio of immune-related targets, supporting diagnostic developers and researchers. Inquire about customized sourcing for rare targets in the innate immunity pathway.