Anti-IL4 Rabbit Monoclonal Antibody for WB and ELISA - P05112

Core Product Specifications and Parameters

Biological Background: IL4 Function and Localization

• IL4 is a cytokine secreted by mast cells, T-cells, eosinophils, and basophils that governs antibody production, hematopoiesis, inflammation, and T-cell responses. Related references: PMID:1993171 PMID:3016727
• It induces class II MHC expression on resting B-cells and enhances secretion and surface expression of IgE and IgG1. Related references: PMID:1993171
• IL4 upregulates the low-affinity IgE receptor (CD23) on lymphocytes and monocytes, further modulating allergic and inflammatory pathways. Related references: PMID:2521231
• It signals through two receptor complexes: type 1 (IL4R and IL2RG) mainly on hematopoietic cells, and type 2 (IL4R and IL13RA1) on nonhematopoietic cells, both activating JAK-STAT6 pathways. Related references: PMID:10219247 PMID:11526337 PMID:18243101 PMID:7721895
• Beyond immunity, IL4 plays a critical role in brain functions such as memory and learning (By similarity).
• The protein undergoes alternative splicing and contains disulfide bonds and glycosylation, which are essential for its stability and activity.
• As a secreted protein, IL4 acts locally and systemically to promote B-cell activation and growth, consistent with its classification as a B-cell activation factor and growth factor.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the mature IL4 sequence (aa 25-153), so the antibody is expected to recognize the secreted cytokine; consider using brefeldin A-treated cell cultures for intracellular detection if exploring other applications.
• For Western blot, validate detection in stimulated immune cell supernatants or lysates; a specific band at approximately 17 kDa should be observed under reducing conditions.
• In ELISA applications, this antibody may serve as a capture or detection reagent; perform checkerboard titrations with recombinant IL4 protein to determine optimal working concentrations.
• Cross-reactivity is confirmed for human IL4; evaluate performance in other species if needed, as the antibody may not recognize mouse or rat IL4.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive suite of IVD raw materials, including this anti-IL4 antibody, to support immune function and allergy research. We offer matched antibody pairs, recombinant antigens, and bulk ancillary reagents to accelerate assay development, as well as custom sourcing for rare specificity targets. Partner with us to streamline your diagnostic pipeline from concept to clinic.