Anti-IL8 Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, FC, ELISA - P10145

Core Product Specifications and Parameters

Biological Background: IL8 Function and Localization

• Interleukin-8 (IL-8, CXCL8), also designated as neutrophil-activating protein 1 (NAP-1), monocyte-derived neutrophil chemotactic factor (MDNCF), and granulocyte chemotactic protein 1 (GCP-1), is a member of the CXC chemokine family.
• IL-8 functions as a potent chemoattractant for neutrophils, basophils, and T-cells, playing a central role in the inflammatory response to clear pathogens and protect the host from infection. Related references: PMID:18692776 PMID:7636208
• It is critically involved in neutrophil activation, enhancing their effector functions. Related references: PMID:2145175 PMID:9623510
• IL-8 signals through binding to the G protein-coupled receptors CXCR1 and CXCR2, which are primarily expressed on neutrophils, monocytes, and endothelial cells, leading to activation of downstream PI3K and MAPK pathways. Related references: PMID:1840701 PMID:1891716 PMID:11971003 PMID:8662698
• Subcellularly, IL-8 is secreted into the extracellular space, consistent with its role as a signaling molecule.
• Post-translational modifications, including citrullination, have been noted for IL-8.
• The mature form of IL-8 has a molecular weight of approximately 11 kDa.

Experimental Guidance and Technical Tips

• When performing Western blotting, a band around 11 kDa corresponding to mature IL-8 can be expected; consider using reducing conditions and appropriate molecular weight markers.
• For immunohistochemistry (IHC-P), antigen retrieval may enhance detection; validate the staining pattern in relevant inflammatory or tumor tissue sections.
• In immunofluorescence (IF/ICC), since IL-8 is secreted, intracellular staining (following permeabilization) or co-staining with Golgi markers may be useful to capture the protein in producer cells.
• For flow cytometry (FC), use intracellular staining protocols; include appropriate isotype controls and validate with known IL-8-expressing cell lines after stimulation.
• In ELISA applications, the antibody may be suitable as a capture or detection reagent; optimize pairing with other antibodies for best sensitivity.
• As the immunogen corresponds to amino acids 28-99, the antibody likely recognizes the mature secreted form; verify specificity in relevant biological context.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your streamlined partner for sourcing IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and assistance with rare target procurement. For researchers and manufacturers developing assays around inflammation markers such as IL-8, we provide comprehensive support from concept to clinic. Contact our team to explore how we can accelerate your diagnostic development with reliable, high-quality raw materials.