Anti-Islet1 Monoclonal Antibody for WB, IHC-P, ELISA - P61371

Core Product Specifications and Parameters

Biological Background: Islet1 Function and Localization

• Islet1 (ISL1) is a DNA-binding transcriptional activator that recognizes the consensus octamer motif 5'-ATAATTAA-3' in target gene promoters, playing a central role in gene regulatory networks.
• It is essential for retinal ganglion cell differentiation, where it cooperates with POU4F2 to achieve maximal expression of RGC target genes.
• Together with LHX3 and LDB1, Islet1 is involved in the specification of motor neurons.
• It binds to insulin gene enhancer sequences, contributing to pancreatic islet cell development (by similarity).
• Islet1 is a critical factor in heart development, marking a progenitor cell population that contributes to the outflow tract, right ventricle, and atrial cells, and is required for cardiac progenitor migration and proliferation via control of FGF and BMP growth factors.
• Subcellular localization is predominantly nuclear.
• It is expressed in subsets of neurons (adrenal medulla, dorsal root ganglion), retinal inner nuclear and ganglion cell layers, the pineal gland, and specific brain regions.
• Belongs to the LIM-homeodomain family; contains two N-terminal LIM domains (zinc-binding) and a homeobox DNA-binding domain. Post-translational modifications include phosphorylation and ubiquitination.

Experimental Guidance and Technical Tips

• The immunogen lies within a central region (aa 200-300) of human Islet1, which is well-conserved in mouse, supporting the observed cross-reactivity; this sequence is also present in known isoforms, so the antibody may detect multiple splice variants if expressed.
• For Western blot (WB), expect a major band near 39 kDa; reducing conditions and standard SDS-PAGE are suitable.
• For immunohistochemistry (IHC-P), nuclear staining is anticipated; preliminary testing of antigen retrieval methods (e.g., heat-induced epitope retrieval at pH 6 or 9) and antibody titration is recommended.
• For ELISA, the antibody can be used for either direct or indirect detection of the immunizing peptide or full-length protein; consider pairing with a suitable secondary antibody.
• Given the developmental and tissue-specific nature of Islet1, validation of specificity in relevant positive controls (e.g., retinal or cardiac tissue, motor neuron cultures) and negative controls (Islet1 knockout/knockdown) is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents for targets like Islet1 in developmental biology and oncology. Our sourcing capabilities extend to rare targets and custom antibody production, backed by technical consulting from concept to clinic. For anti-Islet1 antibody needs, CamelBio provides reliable access to high-quality reagents and tailored solutions.