Anti-IL7 Rabbit Monoclonal Antibody for WB, ELISA - P13232

Core Product Specifications and Parameters

Biological Background: IL-7 Function and Localization

• Interleukin-7 (IL-7) is a hematopoietic cytokine essential for the development, expansion, and survival of naive and memory T-cells and B-cells, thereby maintaining lymphoid homeostasis (PubMed:25870237, PubMed:7527823). Related references: PMID:25870237 PMID:7527823

• IL-7 signals through a receptor complex composed of the IL-7 receptor alpha subunit (IL7RA) and the common gamma chain (CSF2RG), leading to the recruitment and activation of JAK1 and JAK3 kinases (PubMed:8128231). Related references: PMID:8128231

• Downstream signaling pathways activated by IL-7 include the PI3K/Akt/mTOR pathway and the JAK-STAT5 pathway, which mediate cell survival and proliferation (PubMed:18523275, PubMed:20974963). Related references: PMID:18523275 PMID:20974963

• IL-7 is a secreted protein, consistent with its role as an extracellular cytokine; it undergoes alternative splicing to produce multiple isoforms.

• The protein is a glycoprotein with a predicted molecular weight of approximately 20 kDa, containing intramolecular disulfide bonds critical for its three-dimensional structure.

• The UniProt record for IL-7 includes the keyword 'Disease variant', indicating that genetic variations in IL7 may contribute to pathological conditions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 26-177 of human IL-7, which covers the mature protein sequence excluding the signal peptide. This design is expected to recognize the secreted, biologically active form.
• For Western blot, a band around 20 kDa may be observed; however, post-translational modifications such as glycosylation can alter the apparent molecular weight. Consider using deglycosylation enzymes or appropriate positive controls for validation.
• The antibody is suitable for ELISA; ensure the coating antigen presents the epitope in its native conformation, as the antibody was generated against a recombinant protein.
• Test cross-reactivity with other species if needed; this product is reported to react with human IL-7, and sequence homology suggests potential cross-reactivity with non-human primates, but empirical validation is required.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive sourcing partner for IVD raw materials, offering a one-stop solution from concept to clinic. This IL-7 rabbit monoclonal antibody is part of our extensive portfolio of validated antibodies for diagnostic development in immunology and immuno-oncology. We supply optimized antibody pairs, bulk reagents, and rare target materials to accelerate your assay development.