Anti-HLA-DRB1 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P01911

Core Product Specifications and Parameters

Biological Background: HLA-DRB1 Function and Localization

• HLA-DRB1 encodes the beta chain of the MHC class II molecule, which pairs with the alpha chain HLA-DRA to present antigenic peptides to CD4+ T cells, orchestrating adaptive immune responses.
• The peptide-binding groove typically accommodates extracellular antigens of 10-30 amino acids derived from endocytosed proteins, with a key anchor residue at position 1 (PubMed:8145819). Related references: PMID:8145819
• In addition to exogenous antigen presentation, HLA-DRB1 can present intracellular peptides via autophagy, important for thymic T cell selection and central tolerance (PubMed:17182262, PubMed:23783831). Related references: PMID:17182262 PMID:23783831
• Subcellularly, the protein localizes to the cell membrane, endoplasmic reticulum, and endolysosomal compartments (late endosomes, lysosomes, autolysosomes), reflecting its trafficking and antigen-processing roles.
• Tissue expression is predominantly in professional antigen-presenting cells: monocyte/macrophages, dendritic cells, and B cells, as well as in thymic epithelial cells (PubMed:19830726, PubMed:23783831, PubMed:31495665). Related references: PMID:19830726 PMID:23783831 PMID:31495665
• HLA-DRB1 is highly polymorphic, with hundreds of allelic variants; specific alleles are associated with presentation of distinct pathogen-derived epitopes, influencing susceptibility to infectious diseases and autoimmunity (PubMed:22327072, PubMed:29884618, PubMed:27591323). Related references: PMID:22327072 PMID:29884618 PMID:27591323
• The protein undergoes post-translational modifications including glycosylation and disulfide bond formation, and also serves as a receptor for Epstein-Barr virus entry into lymphocytes.
• Key biological keywords include adaptive immunity, MHC II, glycoprotein, and transmembrane protein, highlighting its central role in immune recognition and signaling.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning residues 100–200 of human HLA-DRB1 (P01911). This region may include portions of the beta-2 domain; consider its conservation across alleles when interpreting cross-reactivity in experiments.
• For Western blotting (WB), the predicted molecular weight is approximately 30 kDa; validate with appropriate positive controls (e.g., lymphoblastoid cell lines or isolated peripheral blood mononuclear cells) under reducing conditions.
• In immunohistochemistry (IHC-P), antigen retrieval methods (e.g., heat-induced epitope retrieval in citrate buffer) may be necessary to expose the epitope in formalin-fixed paraffin-embedded tissues.
• For ELISA applications, the antibody can be used as a capture or detection reagent; optimize coating conditions and use recombinant HLA-DRB1 protein or peptide-HLA complexes as standards.
• Given the polymorphism of HLA-DRB1, confirm allele-specific reactivity if needed using engineered cell lines expressing defined alleles or by sequencing.
• As with any monoclonal antibody, perform serial dilutions and include isotype controls to ensure specificity and minimize background.

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