Anti-Hex Rabbit Monoclonal Antibody for WB, ELISA - Q03014

Core Product Specifications and Parameters

Biological Background: Hex (HHEX) Function and Localization

• HHEX (also known as Hex or PRH) is a homeobox protein that acts as a transcriptional repressor by binding the DNA sequence 5'-ATTAA-3'.
• It functions as an activator of WNT-mediated transcription when in complex with CTNNB1 (beta-catenin).
  Related references: PMID:20028982
• HHEX establishes anterior identity during embryonic development by enhancing canonical WNT signaling (via TLE4 repression) and inhibiting NODAL signaling.
• It inhibits EIF4E-mediated mRNA nuclear export, implicating it in post-transcriptional regulation.
  Related references: PMID:12554669
• HHEX may play a role in hematopoietic differentiation.
  Related references: PMID:8096636
• It localizes primarily to the nucleus, including nuclear bodies, but can also be detected in the cytoplasm.
• The protein is classified as a phosphoprotein and is subject to proteolytic processing (Keywords: Phosphoprotein, Proteomics identification).
• Tissue expression is notable in the liver and the promyelocytic leukemia cell line HL-60, consistent with its role in hepatic and hematopoietic systems.

Experimental Guidance and Technical Tips

• Since the immunogen is a synthetic peptide spanning residues 50–150, the antibody likely recognizes the N-terminal region; consider using a blocking peptide to confirm specificity.
• For Western blot, the predicted molecular weight is ~30 kDa; however, post-translational modifications may cause slight band shifts—validate in your sample system.
• This monoclonal antibody is suitable for ELISA; consider pairing with a capture antibody for sandwich ELISA development if needed.
• Cross-reactivity has been validated only with human; for other species, check sequence homology and verify experimentally.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to IVD raw materials, technical support, and supply chain solutions. Alongside this Hex monoclonal antibody, we offer validated antibody pairs, optimized reagents, and rare target sourcing—all designed to support your transcription factor research and assay development from concept to clinic.