Anti-IL2 Rabbit Monoclonal Antibody for WB, FC, ELISA - P60568

Core Product Specifications and Parameters

Biological Background: IL2 Function and Localization

• Interleukin-2 (IL-2), also known as T-cell growth factor (TCGF) and lymphokine, is a critical immunoregulatory cytokine.
• Produced predominantly by activated CD4+ helper T-cells, and to a lesser extent by CD8+ T-cells and natural killer (NK) cells, playing a pivotal role in immune response and tolerance (PubMed:6438535). Related references:
• PMID:6438535
• Binds to a heterotrimeric high-affinity IL-2 receptor (IL-2Rα/CD25, IL-2Rβ/CD122, γc/CD132) or a dimeric intermediate-affinity receptor (IL-2Rβ, γc) (PubMed:16293754, PubMed:16477002). Related references:
• PMID:16293754
• PMID:16477002
• Receptor engagement activates JAK1/JAK3 kinases, leading to phosphorylation and nuclear translocation of STAT5, and stimulates the PI3K/AKT and MAPK signaling cascades (PubMed:7973659, PubMed:8580378, PubMed:25142963). Related references:
• PMID:7973659
• PMID:8580378
• PMID:25142963
• Functions as a potent T-cell growth factor and enhances the cytolytic activity of NK cells (PubMed:6608729). Related references:
• PMID:6608729
• Promotes strong proliferation of activated B-cells and subsequent immunoglobulin production (PubMed:6438535). Related references:
• PMID:6438535
• Controls the survival and proliferation of regulatory T-cells (Tregs), which are essential for the maintenance of immune tolerance.
• Participates in the differentiation and homeostasis of effector T-cell subsets, including Th1, Th2, Th17, and memory CD8+ T-cells.
• The protein is secreted as a glycoprotein and contains an N-terminal signal peptide and intrachain disulfide bonds (UniProt: P60568).

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein containing the full-length mature human IL-2 sequence (amino acids 1–153). This design covers the complete extracellular domain and may recognize both glycosylated and unglycosylated protein forms.
• For Western blotting (WB), a band around 18 kDa is expected; however, glycosylation and sample preparation can cause apparent size shifts. Include appropriate positive controls and molecular weight markers.
• IL-2 is a secreted cytokine; for flow cytometry (FC), intracellular staining with a protein transport inhibitor (e.g., brefeldin A or monensin) is recommended to trap the protein inside activated cells.
• This antibody could be evaluated as a capture or detection reagent in sandwich ELISA formats when paired with a validated antibody targeting a non-overlapping epitope. Titration and cross-reactivity testing are advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with streamlined access to premium IVD raw materials, technical services, and consulting across the entire development pipeline. This anti-IL2 rabbit monoclonal antibody exemplifies our commitment to supplying high-performance reagents for immunoassay development, immune monitoring, and cytokine research. In addition to individual antibodies, we offer validated antibody pairs, bulk reagents, and specialized sourcing for rare targets to help accelerate your next project from concept to clinic.