Anti-ERCC5 Polyclonal Antibody for IF/ICC, ELISA - P28715

Core Product Specifications and Parameters

Biological Background: DNA Excision Repair Protein ERCC-5 (XPG) Function and Localization

• ERCC5 (XPG) is a single-stranded structure-specific DNA endonuclease that makes the 3' incision in nucleotide excision repair (NER). Related references: PMID:32522879, PMID:32821917, PMID:7651464, PMID:8078765

• ERCC5 binds and bends DNA repair bubble substrates and disrupts base stacking at single-strand/double-strand DNA junctions. Related references: PMID:32522879

• It participates in base excision repair (BER) by enhancing the binding and catalytic activity of the DNA glycosylase NTHL1 for removal of oxidized pyrimidines. Related references: PMID:9927729

• ERCC5 is critical for transcription-coupled NER (TCR), cooperating with ERCC6/CSB to recognize stalled RNA polymerase II and stimulating ERCC6/CSB’s binding and ATPase activity. Related references: PMID:16246722

• The protein contributes to replication fork maintenance and genomic stability, also playing roles in homologous recombination repair (HRR) by recruiting RAD51, BRCA2, and PALB2, and by stabilizing stalled forks. Related references: PMID:26833090, PMID:32522879

• Within the TFIIH complex, ERCC5 stimulates the helicase activity of XPD/ERCC2 and the translocase activity of XPB/ERCC3. Related references: PMID:31253769

• ERCC5 localizes predominantly to the nucleus and chromosomes.

• Mutations in ERCC5 cause xeroderma pigmentosum group G (XPG) and are also linked to Cockayne syndrome.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–270) of human ERCC5; this design may enable recognition of full-length protein and some isoforms. Validate in the relevant sample system.
• For immunofluorescence (IF/ICC), optimize fixation and permeabilization protocols to preserve nuclear structure, as ERCC5 is a nuclear protein.
• In ELISA applications, consider using a compatible coating buffer and perform a checkerboard titration to determine optimal antibody and antigen concentrations.
• Confirm cross-reactivity in mouse and rat samples by comparing signal intensities to a human positive control; expression levels may vary between tissues.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated partner for diagnostic manufacturers and research laboratories, offering streamlined access to a broad portfolio of IVD raw materials. This ERCC5 polyclonal antibody exemplifies our commitment to supplying validated tools for DNA repair research, with options for custom formulations, bulk ordering, and complementary reagents such as validated antibody pairs and ancillary proteins. Whether you need optimized monoclonal/polyclonal antibodies or rare-target sourcing, CamelBio bridges your sourcing gaps from concept to clinic.