Anti-Kallikrein-5 (KLK5) Polyclonal Antibody for WB and ELISA - Q9Y337

Core Product Specifications and Parameters

Biological Background: Kallikrein-5 Function and Localization

• Kallikrein-5 (KLK5) is a secreted serine protease belonging to the tissue kallikrein family, also known as stratum corneum tryptic enzyme (SCTE) or KLK-L2.
• It plays a role in skin desquamation by cleaving corneodesmosomal proteins, facilitating the shedding of corneocytes from the skin surface.
• KLK5 is a glycoprotein with conserved disulfide bonds, synthesized as a preproenzyme and activated by proteolytic cleavage.
• It is predominantly expressed in the stratum granulosum of palmar skin, with additional expression in breast, brain, and testis.
• The mature protein has a predicted molecular weight of approximately 32 kDa, consistent with its observed migration on SDS-PAGE.
• As a member of the S1 serine protease family, KLK5 may participate in epidermal proteolytic cascades regulating barrier function.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 41-177 of human KLK5, covering the catalytic domain; consider validating antibody specificity using recombinant KLK5 or tissue lysates known to express the target.
• For Western blot, reducing conditions and a molecular weight marker around 32 kDa are recommended; verify band identity with appropriate controls.
• Given the polyclonal nature, this antibody may offer enhanced sensitivity for ELISA; titrate the antibody and test in the sample matrix of interest.
• Cross-reactivity with mouse and rat suggests epitope conservation; validate performance in these species before use in comparative studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw material procurement by offering comprehensive access to validated antibodies, bulk reagents, and technical support from concept to clinic. This anti-KLK5 antibody supports research into skin biology and protease-related diagnostics, complementing our supply capabilities for rare-target sourcing, antibody pair development, and ancillary reagent provision. Partner with CamelBio to accelerate your diagnostic development with reliable, tailored raw-material solutions.