Anti-ESRRA (ERR-alpha) Rabbit Polyclonal Antibody - P11474

Core Product Specifications and Parameters

Biological Background: ESRRA Function and Localization

• ESRRA (ERR-alpha) is a nuclear receptor that functions as a ligand-dependent transcription factor.
• It binds to estrogen-related receptor response elements (ERRE) containing the consensus half-site 5'-TNAAGGTCA-3' and regulates genes involved in energy metabolism, such as medium-chain acyl-CoA dehydrogenase (MCAD) and lactoferrin.
• Requires dimerization and the coactivator PGC-1A (PPARGC1A) for full transcriptional activity, forming a key complex in metabolic regulation.
• Induces the expression of PERM1 in skeletal muscle, linking ESRRA to muscle energy homeostasis.
• Localizes primarily to the nucleus but can also be detected in the cytoplasm.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitination, which may modulate its activity.
• Belongs to the nuclear receptor subfamily 3 group B (NR3B1), also known as estrogen receptor-like 1 (ESRL1) or ERR-alpha.
• Contains zinc-finger DNA-binding domains and is a metal-binding receptor involved in transcription regulation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-76) of human ESRRA, suggesting the antibody may recognize epitopes in this domain; validate reactivity with relevant isoforms.
• For Western blotting, a band of approximately 46 kDa is expected under reducing conditions; confirm molecular weight in your lysate.
• When performing immunocytochemistry (IF/ICC) or immunohistochemistry (IHC-P), use fixation and permeabilization protocols that preserve nuclear antigens.
• Test cross-reactivity with mouse and rat models, as indicated, but always validate in your specific tissue or cell line.
• The polyclonal nature may yield lower background if pre-adsorbed or used with optimized blocking buffers; consider concentration gradients for ELISA.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing bridge for IVD raw materials, providing diagnostic manufacturers and researchers with validated antibodies, bulk reagents, and customized support. For nuclear receptor targets like ESRRA, we offer high-quality polyclonal antibodies, antibody pairs, and ancillary materials to streamline assay development and scale-up. Tap into our global sourcing network to meet your specific raw material needs, from research to clinical applications.