Anti-EPOR Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P19235

Core Product Specifications and Parameters

Biological Background: EPOR (Erythropoietin Receptor) Function and Localization

• The EPOR gene encodes the erythropoietin receptor, a transmembrane protein that binds erythropoietin to drive erythroblast proliferation and differentiation. Ligand-induced dimerization triggers JAK2/STAT5 signaling (PubMed:10388848, PubMed:2163695, PubMed:2163696), and in some contexts, STAT1 and STAT3 are activated (PubMed:11756159). Related references: PMID:10388848 PMID:2163695 PMID:2163696 PMID:11756159
• A truncated soluble isoform functions as a dominant-negative receptor, attenuating EPOR-mediated signaling.
• Multiple isoforms are generated by alternative splicing; EPOR-F is the predominant form in late-stage erythroid progenitors, while EPOR-S and EPOR-T are abundant in bone marrow, with EPOR-T being the most abundant in early-stage erythroid progenitors.
• The receptor localizes primarily to the cell membrane; a secreted isoform has also been identified.
• Post-translational modifications include glycosylation, phosphorylation, disulfide bond formation, and ubiquitination.
• Mutations in EPOR are associated with familial erythrocytosis (polycythemia).
• The predicted molecular weight of the full-length protein is approximately 55 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 274–508 within the intracellular domain of human EPOR; consider permeabilization steps for immunofluorescence applications to ensure antibody access to this epitope.
• For Western blot, anticipate a band around 55 kDa in lysates from erythroid lineage cells or EPOR-expressing tissues; validate specificity using appropriate positive and negative controls.
• This polyclonal antibody is reactive with human, mouse, and rat EPOR; verify cross-reactivity in your experimental system if using other species.
• As a polyclonal antibody, lot-to-lot consistency may vary; we recommend titrating the antibody for each new batch and application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to high-quality IVD antibodies and ancillary raw materials. Our EPOR polyclonal antibody supports erythropoietin receptor research, with validated applications in WB, IF/ICC, IF-P, and ELISA, and cross-reactivity with human, mouse, and rat. Whether you require bulk antibody supplies, custom formulations, or rare target sourcing, CamelBio accelerates your development from concept to clinic.