Anti-EREG Rabbit Polyclonal Antibody for WB, ELISA - O14944

Core Product Specifications and Parameters

Biological Background: EREG Function and Localization

• Proepiregulin is the precursor of epiregulin (EREG), a ligand of the epidermal growth factor receptor (EGFR) and ERBB4. Related references: PMID:9419975
• It stimulates tyrosine phosphorylation of EGFR and ERBB4. Related references: PMID:9419975
• EREG contributes to inflammation, wound healing, tissue repair, and oocyte maturation by regulating angiogenesis, vascular remodeling, and cell proliferation. Related references: PMID:24631357
• The protein is located in the extracellular space and at the cell membrane (secreted and cell membrane forms).
• In normal adults, EREG is predominantly expressed in the placenta and peripheral blood leukocytes, with high levels detected in carcinomas of the bladder, lung, kidney, and colon.
• Keywords associated with EREG include angiogenesis, mitogen, developmental protein, disulfide bond, glycoprotein, and growth factor.

Experimental Guidance and Technical Tips

• For Western blot, a band migrating around 19 kDa should be detected, consistent with the predicted molecular weight. Given the polyclonal nature, it is advisable to validate specificity using appropriate controls (e.g., blocking peptide or knockout samples).
• In ELISA, optimize coating conditions and antibody dilutions to achieve optimal signal-to-noise ratios. Pair with validated detection reagents for sandwich assays.
• The antibody cross-reacts with human, mouse, and rat EREG; confirm reactivity in your species and tissue of interest using positive controls (e.g., placenta lysate or cancer cell lines).
• Consider potential post-translational modifications (glycosylation, disulfide bonds) that may affect observed molecular weight or antigen recognition.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a comprehensive sourcing solution for IVD raw materials. This anti-EREG polyclonal antibody complements our portfolio for EGFR signaling and cancer research. We also offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to accelerate your assay development from concept to clinic.