Anti-PROCR (EPCR) Rabbit Polyclonal Antibody for WB and ELISA - Q9UNN8

Core Product Specifications and Parameters

Biological Background: PROCR Function and Localization

• PROCR, also known as endothelial protein C receptor (EPCR), is a transmembrane receptor encoded by the PROCR gene in humans.
• It binds activated protein C with high affinity and enhances its activation by the thrombin-thrombomodulin complex, playing a crucial role in the protein C anticoagulant pathway.
• PROCR is primarily localized on the plasma membrane of endothelial cells, consistent with its role in regulating coagulation at the vascular surface.
• Tissue expression is strongest in large-vessel endothelium of arteries and veins in the heart and lung, with lower levels in capillaries, and it is absent from small vessels of the liver and kidney.
• The receptor contains a single transmembrane domain, a signal peptide, and disulfide bonds, and it undergoes N-glycosylation (as indicated by its glycoprotein annotation).
• PROCR is also involved in cytoprotective and anti-inflammatory signaling, though its core function remains in hemostasis.

Experimental Guidance and Technical Tips

• The immunogen used was a recombinant protein covering amino acids 18-210 of human PROCR, suggesting that the antibody likely recognizes the extracellular domain; confirm epitope recognition in your target application.
• For Western blot, the predicted molecular weight is ~27 kDa; however, glycosylation and other post-translational modifications may cause band shifts; validate with appropriate controls and molecular weight markers.
• In ELISA applications, consider using a recombinant PROCR protein as a positive control and optimizing antibody dilution.
• Cross-reactivity with mouse PROCR is reported; when using mouse samples, validate specificity under your experimental conditions, as sequence homology may lead to detection of the murine receptor.

CamelBio: Your One-Stop Sourcing Bridge

At CamelBio, we offer comprehensive sourcing solutions for IVD raw materials, supporting manufacturers and laboratories from concept to clinic. This anti-PROCR polyclonal antibody is an excellent choice for coagulation research and assay development, and we can supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For rare or custom targets, our dedicated team assists with procurement to accelerate your diagnostic development.