Anti-ERC2 Polyclonal Antibody for WB, IHC-P, ELISA - O15083

Core Product Specifications and Parameters

Biological Background: ERC2 Function and Localization

• ERC2 (ERC protein 2) is encoded by the ERC2 gene and is also known by aliases CAST, CAST1, ELKSL, Spc110, and SPBC110.
• It is thought to be involved in organizing the cytomatrix at the nerve terminals active zone (CAZ), a specialized region critical for neurotransmitter release.
• ERC2 likely functions in concert with BSN (Bassoon) to scaffold and regulate presynaptic protein complexes.
• The protein may recruit liprin-alpha proteins to the CAZ, contributing to synaptic vesicle docking and priming.
• Subcellular localization studies indicate presence in the cytoplasm, at synapses, specifically the presynaptic active zone, and associated with the cytoskeleton.
• Structural features include coiled-coil regions, and ERC2 is subject to phosphorylation (as a phosphoprotein).
• It is classified as a reference proteome component and is primarily associated with neuronal cell projections and synaptic function.
• No disease associations are directly annotated, but its role in synapse organization makes it relevant to neurological research.

Experimental Guidance and Technical Tips

• The immunogen targets a region within amino acids 770–850 of human ERC2 (NP_056391.1). Epitope accessibility should be considered when selecting applications; denaturing conditions (e.g., WB) may differ from native conformations (e.g., IHC-P).
• For Western blotting, a band around 111 kDa is expected. Include appropriate positive controls such as human, mouse, or rat brain lysates, as ERC2 is neuronally expressed.
• In immunohistochemistry (paraffin sections, IHC-P), optimize antigen retrieval protocols to uncover the epitope, and verify staining patterns in brain tissue sections.
• ELISA performance will depend on coating antigen and detection conditions; preliminary checkerboard titrations are recommended to establish optimal working concentrations.
• When extending to mouse or rat models, verify sequence conservation in the immunogen region to ensure specificity; cross-reactivity is reported for these species.

CamelBio: Your One-Stop Sourcing Bridge

• As a dedicated IVD raw material partner, CamelBio supports diagnostic manufacturers and research labs with a comprehensive portfolio including validated antibodies, bulk reagents, and specialized sourcing for neuroscience targets. This anti-ERC2 polyclonal antibody fits within our expanding range of synaptic biology tools, complemented by our ability to provide matched antibody pairs, ancillary reagents, and custom production services to streamline your development pipeline.