Anti-DNAJC10 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q8IXB1

Core Product Specifications and Parameters

Biological Background: DNAJC10 Function and Localization

• DNAJC10 (ERdj5, MTHr, JPDI, PDIA19) is an ER-resident disulfide reductase of the DnaJ/Hsp40 family, featuring multiple thioredoxin-like domains and a classical J-domain.
• It works in concert with BiP/GRP78 to uphold ER protein quality control, facilitating both correct folding and disposal of misfolded polypeptides. Related references: PMID:12411443, PMID:23769672, PMID:37739037
• By reducing non-native disulfide bonds in nascent clients, DNAJC10 drives productive oxidative folding. Related references: PMID:23769672, PMID:37739037
• In ER‑associated degradation (ERAD), it selectively reduces incorrect disulfides in EDEM1‑recognized glycoproteins, enabling retrotranslocation and degradation. Related references: PMID:18400946
• Upon ER stress, DNAJC10 promotes pro-apoptotic signals, linking protein misfolding to cell death pathways. Related references: PMID:19122239
• It resides in the ER lumen, where it engages substrate proteins in a redox-dependent manner.
• Alternative splicing yields multiple isoforms, potentially modulating ER homeostasis.

Experimental Guidance and Technical Tips

• The immunogen spans residues 614–793, targeting a region downstream of the catalytic thioredoxin domains; it is expected to recognize full‑length DNAJC10 and may detect certain splice variants.
• For Western blot (WB), a band around 91 kDa is predicted under reducing conditions; verify specificity using appropriate positive and negative lysates.
• In immunofluorescence/immunocytochemistry (IF/ICC), expect a reticular ER‑like staining; optimize fixation and permeabilization to preserve organelle morphology.
• For ELISA applications, the antibody can be used as a capture or detection reagent; titrate against the recombinant immunogen or cell lysates.
• Consider cross‑reactivity with mouse DNAJC10 when designing experiments; validate in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, laboratories, and research institutes with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare or challenging targets. For ER protein folding and stress‑response research, we offer ready‑to‑use reagents and flexible supply solutions to streamline your development from concept to clinic.