Anti-ERK5 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q13164

Core Product Specifications and Parameters

Biological Background: ERK5 Function and Localization

• ERK5 (also known as Big MAP kinase 1 (BMK-1) or MAPK7) is a serine/threonine-protein kinase belonging to the MAP kinase family that plays a critical role in cellular proliferation, differentiation, and survival.
• It is specifically activated by the upstream MAP2K5 (MEK5) kinase, triggering its translocation to the nucleus where it phosphorylates transcription factors such as MEF2C.
• EGF stimulation activates ERK5 through a Ras-independent, MAP2K5-dependent pathway, distinct from the classic ERK1/2 signaling cascade.
• In cardiomyocytes, ERK5 acts as a negative regulator of apoptosis by interacting with STUB1/CHIP to promote ubiquitination and degradation of pro-apoptotic ICER isoforms of CREM.
• ERK5 contributes to endothelial function and blood vessel integrity, and may be involved in muscle cell differentiation.
• It phosphorylates SGK1 at Ser-78, a modification required for growth factor-induced cell cycle progression.
• Subcellularly, ERK5 is found in the cytoplasm and nucleus, and concentrates in PML nuclear bodies upon activation.
• It is expressed in many adult tissues, with highest abundance in heart, placenta, lung, kidney, and skeletal muscle, but is undetectable in liver.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-310) of human ERK5, which may detect both full-length and any N-terminally truncated isoforms. Validation in the relevant sample system is advised.
• For Western blotting, a band near the predicted molecular weight of 88 kDa should be expected; consider using appropriate positive control lysates (e.g., HeLa, HEK293, or tissue samples with high ERK5 expression such as heart or skeletal muscle).
• In immunohistochemistry (IHC-P) and immunocytochemistry (IF/ICC), optimization of antigen retrieval and permeabilization steps may be required to access nuclear ERK5 pools. Use positive and negative control tissues/cells to confirm staining specificity.
• For ELISA, whether as capture or detection antibody, cross-reactivity with other MAP kinases should be assessed; testing against recombinant ERK1, ERK2, or JNK is recommended to confirm specificity.
• Given reported cross-reactivity with mouse and rat, this antibody is suitable for translational studies across these species, but sequence alignment of the immunogen region is recommended to confirm epitope conservation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing bridge for IVD raw materials, streamlining access to high-quality antibodies, antigens, and reagents for diagnostic and research applications. For MAP kinase signaling targets like ERK5, we supply validated antibody pairs, bulk antibodies, and ancillary reagents to meet your development needs. Whether you require routine raw materials or custom sourcing for rare targets, CamelBio supports your journey from concept to clinic.